LOCUS       sequence-51532-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PDZK1
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(350..370)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(395..412)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             423..445
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    460..484
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(484..503)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(485..503)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(587..606)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        812..889
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 tggtggtggt ggtgctcgag tgcggccgca agcttgtcga cggagctcga attcggatcc
       61 gtatccacct ttactgtcaa accgtatcct gggccttctt tccacagact agaagtgtga
      121 cattcttccc actgctctgg attctatcca ccaccttctc atagggttca tctagcacat
      181 tcaccccatt cacttcaatg atgacatcct catcctctag cccagccaag tcagcaggac
      241 cgcccttctg tacctctttg atgaatgagc ctggcagacc ccgaatcgca tttaagtgaa
      301 agccatagcc attttcacct ttagccagcc tgcagagttt aggcttcatg gattggaagt
      361 acaggttctc ggtacccaga tctacaccag aagaatgatg atgatgatgg tgcatatgta
      421 tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa
      481 ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga
      541 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
      601 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
      661 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
      721 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
      781 gagtcgcata agggagagcg tcgagatccc ggacaccatc gaatggcgca aaacctttcg
      841 cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt
      901 aacg
//
