LOCUS       sequence-51534-e         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PDZK1
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..34
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             370..387
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(440..458)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      454..501
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(625..644)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agatctgggt accgagaacc tgtacttcca atccatgaag cctaaactct gcaggctggc
       61 taaaggtgaa aatggctatg gctttcactt aaatgcgatt cggggtctgc caggctcatt
      121 catcaaagag gtacagaagg gcggtcctgc tgacttggct gggctagagg atgaggatgt
      181 catcattgaa gtgaatgggg tgaatgtgct agatgaaccc tatgagaagg tggtggatag
      241 aatccagagc agtgggaaga atgtcacact tctagtctgt ggaaagaagg cccaggatac
      301 ggtttgacag taaaggtgga tacggatccg aattcgagct ccgtcgacaa gcttgcggcc
      361 gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa
      421 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      481 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg
      541 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      601 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
      661 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
      721 ctttacggc
//
