LOCUS       sequence-51541-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Neerincx A, Rodriguez GM, Steimle V, Kufer TA
  TITLE     NLRC5 Controls Basal MHC Class I Gene Expression in an MHC 
            Enhanceosome-Dependent Manner.
  JOURNAL   J Immunol. 2012 May 15;188(10):4940-50. Epub 2012 Apr 6.
  PUBMED    22490867
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Immunol."; date: "2012-05-15"; volume: "188(10)"; pages: "4940-50. 
            Epub 2012 Apr 6"
COMMENT     Sequence Label: myc-NLRC5 K234A
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             101..130
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             134..163
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             167..196
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             200..223
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 tggctactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
       61 agacccaagc tggctagcgt ttaaacttaa gcttaccatg gagcagaagc tgatctccga
      121 ggaggacctg aacgagcaga agctgatctc cgaggaggac ctgaacgagc agaagctgat
      181 ctccgaggag gacctgaacc tggaggtgct gttccaggga cccggggtac ctaaggatcc
      241 agtgtggtgg aattctgcag atatcatgga ccccgttggc ctccagctcg gcaacaagaa
      301 cctgtggagc tgtcttgtga ggctgctcac caaagaccca gaatggctga acgccaagat
      361 gaagttcttc ctccccaaca cggacctgga ttccaggaac gagaccttgg accctgaaca
      421 gagagtcatc ctgcaactca acaagctgca tgtccagggt tcggacacct ggcagtcttt
      481 cattcattgt gtgtgcatgc agctggaggt gcctctggac ctggaggtgc tgctgctgag
      541 tacttttggc tatgatgatg ggttcaccag ccagctggga gctgagggga aaagccaacc
      601 tgaatctcag ctccaccatg gcctgaagcg cccacatcag agctgtgggt cctcaccccg
      661 ccggaagcag tgcaagaagc agcagctaga gttggccaag aagtacctgc agctcctgcg
      721 gacctctgcc cagcagcgct acaggagcca aatccctggg tcagggcagc cccacgcctt
      781 ccaccaggtc tatgtccctc caatcctgcg ccgggccaca g
//
