LOCUS       sequence-51568-e        8429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Offterdinger M, Bastiaens PI
  TITLE     Prolonged EGFR signaling by ERBB2-mediated sequestration at the 
            plasma membrane.
  JOURNAL   Traffic. 2008 Jan;9(1):147-55. Epub 2007 Nov 13.
  PUBMED    17956594
REFERENCE   2  (bases 1 to 8429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic."; 
            date: "2008-01"; volume: "9(1)"; pages: "147-55. Epub 2007 Nov 13"
COMMENT     Sequence Label: perbB1-Citrine
FEATURES             Location/Qualifiers
     source          1..8429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(718..789,790..2652,2653..2721,2722..4347)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCKLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     CDS             join(4375..4377,4378..4380,4381..5091)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4420..4441)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4681..4700)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5028..5049
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5217..5338
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5254..5273)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5308..5327
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5345..5800)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5482..5503)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5694..5713
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5827..5931
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5933..6290
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5933..6124
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5956..5976)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6141..6276
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6325..7119
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6325..7119
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6379..6398)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6989..7008
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7307..7326)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7351..7398
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7727..8315
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8216..8235
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagagagc
      601 ttatcgattc tagccgagtc cccgcctcgc cgccaacgcc acaaccaccg cgcacggccc
      661 cctgactccg tccagtattg atcgggagag ccggagcgag ctcttcgggg agcagcgatg
      721 cgaccctccg ggacggccgg ggcagcgctc ctggcgctgc tggctgcgct ctgcccggcg
      781 agtcgggctc tggaggaaaa gaaagtttgc caaggcacga gtaacaagct cacgcagttg
      841 ggcacttttg aagatcattt tctcagcctc cagaggatgt tcaataactg tgaggtggtc
      901 cttgggaatt tggaaattac ctatgtgcag aggaattatg atctttcctt cttaaagacc
      961 atccaggagg tggctggtta tgtcctcatt gccctcaaca cagtggagcg aattcctttg
     1021 gaaaacctgc agatcatcag aggaaatatg tactacgaaa attcctatgc cttagcagtc
     1081 ttatctaact atgatgcaaa taaaaccgga ctgaaggagc tgcccatgag aaatttacag
     1141 gaaatcctgc atggcgccgt gcggttcagc aacaaccctg ccctgtgcaa cgtggagagc
     1201 atccagtggc gggacatagt cagcagtgac tttctcagca acatgtcgat ggacttccag
     1261 aaccacctgg gcagctgcca aaagtgtgat ccaagctgtc ccaatgggag ctgctggggt
     1321 gcaggagagg agaactgcca gaaactgacc aaaatcatct gtgcccagca gtgctccggg
     1381 cgctgccgtg gcaagtcccc cagtgactgc tgccacaacc agtgtgctgc aggctgcaca
     1441 ggcccccggg agagcgactg cctggtctgc cgcaaattcc gagacgaagc cacgtgcaag
     1501 gacacctgcc ccccactcat gctctacaac cccaccacgt accagatgga tgtgaacccc
     1561 gagggcaaat acagctttgg tgccacctgc gtgaagaagt gtccccgtaa ttatgtggtg
     1621 acagatcacg gctcgtgcgt ccgagcctgt ggggccgaca gctatgagat ggaggaagac
     1681 ggcgtccgca agtgtaagaa gtgcgaaggg ccttgccgca aagtgtgtaa cggaataggt
     1741 attggtgaat ttaaagactc actctccata aatgctacga atattaaaca cttcaaaaac
     1801 tgcacctcca tcagtggcga tctccacatc ctgccggtgg catttagggg tgactccttc
     1861 acacatactc ctcctctgga tccacaggaa ctggatattc tgaaaaccgt aaaggaaatc
     1921 acagggtttt tgctgattca ggcttggcct gaaaacagga cggacctcca tgcctttgag
     1981 aacctagaaa tcatacgcgg caggaccaag caacatggtc agttttctct tgcagtcgtc
     2041 agcctgaaca taacatcctt gggattacgc tccctcaagg agataagtga tggagatgtg
     2101 ataatttcag gaaacaaaaa tttgtgctat gcaaatacaa taaactggaa aaaactgttt
     2161 gggacctccg gtcagaaaac caaaattata agcaacagag gtgaaaacag ctgcaaggcc
     2221 acaggccagg tctgccatgc cttgtgctcc cccgagggct gctggggccc ggagcccagg
     2281 gactgcgtct cttgccggaa tgtcagccga ggcagggaat gcgtggacaa gtgcaagctt
     2341 ctggagggtg agccaaggga gtttgtggag aactctgagt gcatacagtg ccacccagag
     2401 tgcctgcctc aggccatgaa catcacctgc acaggacggg gaccagacaa ctgtatccag
     2461 tgtgcccact acattgacgg cccccactgc gtcaagacct gcccggcagg agtcatggga
     2521 gaaaacaaca ccctggtctg gaagtacgca gacgccggcc atgtgtgcca cctgtgccat
     2581 ccaaactgca cctacggatg cactgggcca ggtcttgaag gctgtccaac gaatgggcct
     2641 aagatcccgt ccatcgccac tgggatggtg ggggccctcc tcttgctgct ggtggtggcc
     2701 ctggggatcg gcctcttcat gcgaaggcgc cacatcgttc ggaagcgcac gctgcggagg
     2761 ctgctgcagg agagggagct tgtggagcct cttacaccca gtggagaagc tcccaaccaa
     2821 gctctcttga ggatcttgaa ggaaactgaa ttcaaaaaga tcaaagtgct gggctccggt
     2881 gcgttcggca cggtgtataa gggactctgg atcccagaag gtgagaaagt taaaattccc
     2941 gtcgctatca aggaattaag agaagcaaca tctccgaaag ccaacaagga aatcctcgat
     3001 gaagcctacg tgatggccag cgtggacaac ccccacgtgt gccgcctgct gggcatctgc
     3061 ctcacctcca ccgtgcaact catcacgcag ctcatgccct tcggctgcct cctggactat
     3121 gtccgggaac acaaagacaa tattggctcc cagtacctgc tcaactggtg tgtgcagatc
     3181 gcaaagggca tgaactactt ggaggaccgt cgcttggtgc accgcgacct ggcagccagg
     3241 aacgtactgg tgaaaacacc gcagcatgtc aagatcacag attttgggct ggccaaactg
     3301 ctgggtgcgg aagagaaaga ataccatgca gaaggaggca aagtgcctat caagtggatg
     3361 gcattggaat caattttaca cagaatctat acccaccaga gtgatgtctg gagctacggg
     3421 gtgaccgttt gggagttgat gacctttgga tccaagccat atgacggaat ccctgccagc
     3481 gagatctcct ccatcctgga gaaaggagaa cgcctccctc agccacccat atgtaccatc
     3541 gatgtctaca tgatcatggt caagtgctgg atgatagacg cagatagtcg cccaaagttc
     3601 cgtgagttga tcatcgaatt ctccaaaatg gcccgagacc cccagcgcta ccttgtcatt
     3661 cagggggatg aaagaatgca tttgccaagt cctacagact ccaacttcta ccgtgccctg
     3721 atggatgaag aagacatgga cgacgtggtg gatgccgacg agtacctcat cccacagcag
     3781 ggcttcttca gcagcccctc cacgtcacgg actcccctcc tgagctctct gagtgcaacc
     3841 agcaacaatt ccaccgtggc ttgcattgat agaaatgggc tgcaaagctg tcccatcaag
     3901 gaagacagct tcttgcagcg atacagctca gaccccacag gcgccttgac tgaggacagc
     3961 atagacgaca ccttcctccc agtgcctgaa tacataaacc agtccgttcc caaaaggccc
     4021 gctggctctg tgcagaatcc tgtctatcac aatcagcctc tgaaccccgc gcccagcaga
     4081 gacccacact accaggaccc ccacagcact gcagtgggca accccgagta tctcaacact
     4141 gtccagccca cctgtgtcaa cagcacattc gacagccctg cccactgggc ccagaaaggc
     4201 agccaccaaa ttagcctgga caaccctgac taccagcagg acttctttcc caaggaagcc
     4261 aagccaaatg gcatctttaa gggctccaca gctgaaaatg cagaatacct aagggtcgcg
     4321 ccacaaagca gtgaatttat tggagcagcg atcggtggag gtggaggagg caccatggtg
     4381 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     4441 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     4501 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     4561 accaccttcg gctacggcct gatgtgcttc gcccgctacc ccgaccacat gaagcagcac
     4621 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     4681 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4741 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     4801 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     4861 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     4921 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     4981 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     5041 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc
     5101 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     5161 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     5221 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5281 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag
     5341 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     5401 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     5461 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     5521 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     5581 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     5641 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     5701 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     5761 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     5821 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5881 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     5941 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6001 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6061 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6121 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6181 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6241 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     6301 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     6361 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     6421 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     6481 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     6541 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     6601 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     6661 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6721 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6781 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6841 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6901 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6961 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     7021 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     7081 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     7141 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     7201 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     7261 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     7321 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     7381 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     7441 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     7501 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     7561 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7621 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7681 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7741 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7801 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7861 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7921 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7981 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8041 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8101 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8161 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8221 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8281 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8341 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8401 ctgtggataa ccgtattacc gccatgcat
//
