LOCUS       sequence-51570-e        9895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Offterdinger M, Bastiaens PI
  TITLE     Prolonged EGFR signaling by ERBB2-mediated sequestration at the 
            plasma membrane.
  JOURNAL   Traffic. 2008 Jan;9(1):147-55. Epub 2007 Nov 13.
  PUBMED    17956594
REFERENCE   2  (bases 1 to 9895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic."; 
            date: "2008-01"; volume: "9(1)"; pages: "147-55. Epub 2007 Nov 13"
COMMENT     Sequence Label: perbB2-ECFP
FEATURES             Location/Qualifiers
     source          1..9895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4726..4728,4729..4731,4732..5442)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4771..4792)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5032..5051)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5379..5400
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5489..5506)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5495..5719
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5765..6193
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5852..5871)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6062..6083
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6202..6222)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6207..6536
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6387..6522
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6449..6468
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6603..7397
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6657..6676)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7267..7286
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7571..7692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7608..7627)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7662..7681
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7741..7757)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7741..7757)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7754..7776)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7765..7781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7789..7795,7796..7813,7814..7819))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7834..7855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7972..7989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8143..8728)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8223..8242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8899..9690,9691..9759))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9522..9541
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9760..9864)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 accatggagc tggcggcctt gtgccgctgg gggctcctcc tcgccctctt gccccccgga
      961 gccgcgagca cccaagtgtg caccggcaca gacatgaagc tgcggctccc tgccagtccc
     1021 gagacccacc tggacatgct ccgccacctc taccagggct gccaggtggt gcagggaaac
     1081 ctggaactca cctacctgcc caccaatgcc agcctgtcct tcctgcagga tatccaggag
     1141 gtgcagggct acgtgctcat cgctcacaac caagtgaggc aggtcccact gcagaggctg
     1201 cggattgtgc gaggcaccca gctctttgag gacaactatg ccctggccgt gctagacaat
     1261 ggagacccgc tgaacaatac cacccctgtc acaggggcct ccccaggagg cctgcgggag
     1321 ctgcagcttc gaagcctcac agagatcttg aaaggagggg tcttgatcca gcggaacccc
     1381 cagctctgct accaggacac gattttgtgg aaggacatct tccacaagaa caaccagctg
     1441 gctctcacac tgatagacac caaccgctct cgggcctgcc acccctgttc tccgatgtgt
     1501 aagggctccc gctgctgggg agagagttct gaggattgtc agagcctgac gcgcactgtc
     1561 tgtgccggtg gctgtgcccg ctgcaagggg ccactgccca ctgactgctg ccatgagcag
     1621 tgtgctgccg gctgcacggg ccccaagcac tctgactgcc tggcctgcct ccacttcaac
     1681 cacagtggca tctgtgagct gcactgccca gccctggtca cctacaacac agacacgttt
     1741 gagtccatgc ccaatcccga gggccggtat acattcggcg ccagctgtgt gactgcctgt
     1801 ccctacaact acctttctac ggacgtggga tcctgcaccc tcgtctgccc cctgcacaac
     1861 caagaggtga cagcagagga tggaacacag cggtgtgaga agtgcagcaa gccctgtgcc
     1921 cgagtgtgct atggtctggg catggagcac ttgcgagagg tgagggcagt taccagtgcc
     1981 aatatccagg agtttgctgg ctgcaagaag atctttggga gcctggcatt tctgccggag
     2041 agctttgatg gggacccagc ctccaacact gccccgctcc agccagagca gctccaagtg
     2101 tttgagactc tggaagagat cacaggttac ctatacatct cagcatggcc ggacagcctg
     2161 cctgacctca gcgtcttcca gaacctgcaa gtaatccggg gacgaattct gcacaatggc
     2221 gcctactcgc tgaccctgca agggctgggc atcagctggc tggggctgcg ctcactgagg
     2281 gaactgggca gtggactggc cctcatccac cataacaccc acctctgctt cgtgcacacg
     2341 gtgccctggg accagctctt tcggaacccg caccaagctc tgctccacac tgccaaccgg
     2401 ccagaggacg agtgtgtggg cgagggcctg gcctgccacc agctgtgcgc ccgagggcac
     2461 tgctggggtc cagggcccac ccagtgtgtc aactgcagcc agttccttcg gggccaggag
     2521 tgcgtggagg aatgccgagt actgcagggg ctccccaggg agtatgtgaa tgccaggcac
     2581 tgtttgccgt gccaccctga gtgtcagccc cagaatggct cagtgacctg ttttggaccg
     2641 gaggctgacc agtgtgtggc ctgtgcccac tataaggacc ctcccttctg cgtggcccgc
     2701 tgccccagcg gtgtgaaacc tgacctctcc tacatgccca tctggaagtt tccagatgag
     2761 gagggcgcat gccagccttg ccccatcaac tgcacccact cctgtgtgga cctggatgac
     2821 aagggctgcc ccgccgagca gagagccagc cctctgacgt ccatcgtctc tgcggtggtt
     2881 ggcattctgc tggtcgtggt cttgggggtg gtctttggga tcctcatcaa gcgacggcag
     2941 cagaagatcc ggaagtacac gatgcggaga ctgctgcagg aaacggagct ggtggagccg
     3001 ctgacaccta gcggagcgat gcccaaccag gcgcagatgc ggatcctgaa agagacggag
     3061 ctgaggaagg tgaaggtgct tggatctggc gcttttggca cagtctacaa gggcatctgg
     3121 atccctgatg gggagaatgt gaaaattcca gtggccatca aagtgttgag ggaaaacaca
     3181 tcccccaaag ccaacaaaga aatcttagac gaagcatacg tgatggctgg tgtgggctcc
     3241 ccatatgtct cccgccttct gggcatctgc ctgacatcca cggtgcagct ggtgacacag
     3301 cttatgccct atggctgcct cttagaccat gtccgggaaa accgcggacg cctgggctcc
     3361 caggacctgc tgaactggtg tatgcagatt gccaagggga tgagctacct ggaggatgtg
     3421 cggctcgtac acagggactt ggccgctcgg aacgtgctgg tcaagagtcc caaccatgtc
     3481 aaaattacag acttcgggct ggctcggctg ctggacattg acgagacaga gtaccatgca
     3541 gatgggggca aggtgcccat caagtggatg gcgctggagt ccattctccg ccggcggttc
     3601 acccaccaga gtgatgtgtg gagttatggt gtgactgtgt gggagctgat gacttttggg
     3661 gccaaacctt acgatgggat cccagcccgg gagatccctg acctgctgga aaagggggag
     3721 cggctgcccc agccccccat ctgcaccatt gatgtctaca tgatcatggt caaatgttgg
     3781 atgattgact ctgaatgtcg gccaagattc cgggagttgg tgtctgaatt ctcccgcatg
     3841 gccagggacc cccagcgctt tgtggtcatc cagaatgagg acttgggccc agccagtccc
     3901 ttggacagca ccttctaccg ctcactgctg gaggacgatg acatggggga cctggtggat
     3961 gctgaggagt atctggtacc ccagcagggc ttcttctgtc cagaccctgc cccgggcgct
     4021 gggggcatgg tccaccacag gcaccgcagc tcatctacca ggagtggcgg tggggacctg
     4081 acactagggc tggagccctc tgaagaggag gcccccaggt ctccactggc accctccgaa
     4141 ggggctggct ccgatgtatt tgatggtgac ctgggaatgg gggcagccaa ggggctgcaa
     4201 agcctcccca cacatgaccc cagccctcta cagcggtaca gtgaggaccc cacagtaccc
     4261 ctgccctctg agactgatgg ctacgttgcc cccctgacct gcagccccca gcctgaatat
     4321 gtgaaccagc cagatgttcg gccccagccc ccttcgcccc gagagggccc tctgcctgct
     4381 gcccgacctg ctggtgccac tctggaaagg gccaagactc tctccccagg gaagaatggg
     4441 gtcgtcaaag acgtttttgc ctttgggggt gccgtggaga accccgagta cttgacaccc
     4501 cagggaggag ctgcccctca gccccaccct cctcctgcct tcagcccagc cttcgacaac
     4561 ctctattact gggaccagga cccaccagag cggggggctc cacccagcac cttcaaaggg
     4621 acacctacgg cagagaaccc agagtacctg ggtctggacg tgccagtgac cggttctggt
     4681 gggtcaggag ggggcggagg taagcttggt accgagctcg gatccatggt gagcaagggc
     4741 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     4801 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     4861 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     4921 acctggggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     4981 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     5041 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     5101 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     5161 tacatcagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     5221 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     5281 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     5341 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5401 accgccgccg ggatcactct cggcatggac gagctgtaca aggaattcta actcgagtct
     5461 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     5521 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     5581 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     5641 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     5701 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     5761 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5821 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5881 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5941 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6001 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6061 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6121 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6181 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     6241 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     6301 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6361 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6421 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     6481 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6541 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     6601 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     6661 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     6721 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     6781 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     6841 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     6901 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     6961 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     7021 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     7081 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     7141 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     7201 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     7261 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     7321 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     7381 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     7441 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     7501 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     7561 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7621 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7681 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     7741 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7801 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7861 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7921 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7981 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8041 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8101 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8161 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8221 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8281 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8341 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8401 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8461 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8521 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8581 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8641 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca
     8701 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8761 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8821 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8881 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8941 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9001 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9061 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9121 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9181 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9241 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9301 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9361 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9421 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9481 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9541 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9601 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9661 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9721 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9781 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9841 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
