LOCUS       sequence-51575-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3448 (=HL 3425 + pHL 1191)
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     79..97
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    118..136
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    143..161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(217..245)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     840..860
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
       61 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca cctcgagtcc
      121 ctatcagtga tagagattga catccctatc agtgatagag atactgtcga ctaaggagga
      181 aaaaaaatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      241 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      301 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
      361 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
      421 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
      481 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
      541 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
      601 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
      661 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
      721 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
      781 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
      841 gtccttcttg agtttgtaac agctgctggg atacactggc aggaatgg
//
