LOCUS       sequence-51579-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Watt S, Mata J, Lopez-Maury L, Marguerat S, Burns G, Bahler J
  TITLE     urg1: a uracil-regulatable promoter system for fission yeast with 
            short induction and repression times.
  JOURNAL   PLoS One. 2008 Jan 16;3(1):e1428.
  PUBMED    18197241
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2008-01-16"; volume: "3(1)"; pages: "e1428"
COMMENT     Sequence Label: pFA6a-natMX6-Purg1
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(69..266)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(272..844)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
ORIGIN
        1 ggatctgata tcatcgatga attcgagctc gtttaaactg gatggcggcg ttagtatcga
       61 atcgacagca gtatagcgac cagcattcac atacgattga cgcatgatat tactttctgc
      121 gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa aaaaaatata aatcacgcta
      181 acatttgatt aaaatagaac aactacaata taaaaaaact atacaaatga caagttcttg
      241 aaaacaagaa tctttttatt gtcagtactg attaggggca gggcatgctc atgtagagcg
      301 cctgctcgcc gtccgaggcg gtgccgtcgt acagggcggt gtccaggccg cagagggtga
      361 accccatccg ccggtacgcg tggatcgccg gtgcgttgac gttggtgacc tccagccaga
      421 ggtgcccggc accccgctcg cgggcgaact ccgtcgcgag ccccatcaac gcgcgcccga
      481 ccccgtgccc ccggtgctcc ggggcgacct cgatgtcctc gacggtcagc cggcggttcc
      541 agccggagta cgagacgacc acgaagcccg ccaggtcgcc gtcgtccccg tacgcgacga
      601 acgtccgaga gtccgggtcg ccgtcctccc cgtcgtccga ttcgtcgtcc gattcgtcgt
      661 cggggaacac cttggtcagg ggcgggtcca ccggcacctc ccgcagggtg aagccgtccc
      721 cggtggcggt gacgcggaag acggtgtcgg tggtgaagga cccatccagt gcctcgatgg
      781 cctcggcgtc ccccgggaca ctggtgcggt accggtaagc cgtgtcgtca agagtggtac
      841 ccatggttgt ttatgttcgg atgtgatgtg agaactgtat cctagcaaga tttaaaaagg
      901 aagtatatga aagaagaacc tcagtggcaa atcctaacct tttatatttc tctacagggg
      961 cgcggcgtgg ggacaattca acgcgtctgt
//
