LOCUS       sequence-51582-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00473
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(130..150)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
       61 ctttcttata atgccaactt tgtacaagaa agttggcaaa tacgccgtcg ctgcgccggg
      121 atgcatcagg tggtggtggt ggtgatggtg tccgaaatgg ttcagcgagt tcatggcctc
      181 cgcactgccg ctcggcgccg tcgtgtggta ccagtcgctg ggtgggtggt gctgggccgc
      241 cgggtggtac agtaggtggt ggttgtggtg cagcatggag gcggaggcag cagctgcccc
      301 cggagagggc gccgcctggt ggtgatgggg gtggtggtgg tgcgacggcg gcggtcccag
      361 cggcggtggc aggtaggcgg aggcagaagc ggccatcagt tggagggcag gcgaggactt
      421 cgggtcgctg ggatgggcgg tcagctgttg ctgctgctgt gcctgctgct ggtgctgctg
      481 ctgttgttgc tgctgctggt gctggtgctg ctggtgctgc agcaagtggg aagcactgct
      541 gctggccgtg ctgctgctgc cgctgctcga ggctcccagg tggacactgt gcatcggatt
      601 gggctccgac ttgatcgagt gcggggagga cttgatgccg cttccgccgc tcaggctgct
      661 caccccgctg ctgctgcctg cacttccatc cagtcgggtc gaaggcggcg tctgggctcc
      721 gccggtgatg atgctcgagc tggagctctc ctcgccctcg ctgtcgtagc cgtgggatgg
      781 gctcttctcg tccacattgc catgcacctt catgtgcttc cgcagcgaag a
//
