LOCUS       sequence-51590-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3326 (=HL 716 + pHL 1008)
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     75..93
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    114..132
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    139..157
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(213..241)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
       61 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcacctc gagtccctat
      121 cagtgataga gattgacatc cctatcagtg atagagatac tgtcgactaa ggaggaaaaa
      181 aaatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      241 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      301 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      361 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
      421 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
      481 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
      541 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      601 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
      661 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
      721 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
      781 acctgtccac acaatctgcc ctttcgaaag atccccaacg aaaagagaga ccacatg
//
