LOCUS       sequence-51598-e        9860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Mulleder M, Capuano F, Pir P, Christen S, Sauer U, Oliver SG, Ralser
            M
  TITLE     A prototrophic deletion mutant collection for yeast metabolomics and
            systems biology.
  JOURNAL   Nat Biotechnol. 2012 Dec 7;30(12):1176-8. doi: 10.1038/nbt.2442.
  PUBMED    23222782
REFERENCE   2  (bases 1 to 9860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2012-12-7- 7"; volume: "30"; 
            issue: "12"; pages: "1176-8"
COMMENT     Sequence Label: pHLUM
FEATURES             Location/Qualifiers
     source          1..9860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        315..502
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             503..1162
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      complement(1423..1878)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1560..1581)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1772..1791
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2008..2030
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2022..2039
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2023..2039
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2046..2065
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2046..2064
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2097..2113
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2097..2113
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(2591..3685)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(3686..4090)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             complement(4467..5270)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             5667..7001
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFKLSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     promoter        complement(7086..7104)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7125..7141)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7125..7141)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7138..7160)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7149..7165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7173..7179,7180..7197,7198..7203))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(7356..7373)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7527..8115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8286..9077,9078..9146))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8909..8928
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9147..9251)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    9288..9791
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9833..9851
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataattc cgttttaaga gcttggtgag cgctaggagt cactgccagg tatcgtttga
      241 acacggcatt agtcagggaa gtcataacac agtcctttcc cgcaattttc tttttctatt
      301 actcttggcc tcctctagta cactctatat ttttttatgc ctcggtaatg attttcattt
      361 ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat tatcacataa
      421 tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa aaaatgagca
      481 ggcaagataa acgaaggcaa agatgacaga gcagaaagcc ctagtaaagc gtattacaaa
      541 tgaaaccaag attcagattg cgatctcttt aaagggtggt cccctagcga tagagcactc
      601 gatcttccca gaaaaagagg cagaagcagt agcagaacag gccacacaat cgcaagtgat
      661 taacgtccac acaggtatag ggtttctgga ccatatgata catgctctgg ccaagcattc
      721 cggctggtcg ctaatcgttg agtgcattgg tgacttacac atagacgacc atcacaccac
      781 tgaagactgc gggattgctc tcggtcaagc ttttaaagag gccctactgg cgcgtggagt
      841 aaaaaggttt ggatcaggat ttgcgccttt ggatgaggca ctttccagag cggtggtaga
      901 tctttcgaac aggccgtacg cagttgtcga acttggtttg caaagggaga aagtaggaga
      961 tctctcttgc gagatgatcc cgcattttct tgaaagcttt gcagaggcta gcagaattac
     1021 cctccacgtt gattgtctgc gaggcaagaa tgatcatcac cgtagtgaga gtgcgttcaa
     1081 ggctcttgcg gttgccataa gagaagccac ctcgcccaat ggtaccaacg atgttccctc
     1141 caccaaaggt gttcttatgt agtgacaccg attatttaaa gctgcagcat acgatatata
     1201 tacatgtgta tatatgtata cctatgaatg tcagtaagta tgtatacgaa cagtatgata
     1261 ctgaagatga caaggtaatg catcattcta tacgtgtcat tctgaacgag gcgcgctttc
     1321 cttttttctt tttgcttttt cttttttttt ctcttgaact cgacggatca tatgcggtgt
     1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
     1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa ggggggatgt gctgcaaggc
     1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     2041 aattgtaata cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta
     2101 gaactagtgg atcctcgact acgtcgtaag gccgtttctg acagagtaaa attcttgagg
     2161 gaactttcac cattatggga aatgcttcaa gaaggtattg acttaaactc catcaaatgg
     2221 tcaggtcatt gagtgttttt tatttgttgt attttttttt ttttagagaa aatcctccaa
     2281 tatcaaatta ggaatcgtag tttcatgatt ttctgttaca cctaactttt tgtgtggtgc
     2341 cctcctcctt gtcaatatta atgttaaagt gcaattcttt ttccttatca cgttgagcca
     2401 ttagtatcaa tttgcttacc tgtattcctt tactatcctc ctttttctcc ttcttgataa
     2461 atgtatgtag attgcgtata tagtttcgtc taccctatga acatattcca ttttgtaatt
     2521 tcgtgtcgtt tctattatga atttcattta taaagtttat gtacaaatat cataaaaaaa
     2581 gagaatcttt ttaagcaagg attttcttaa cttcttcggc gacagcatca ccgacttcgg
     2641 tggtactgtt ggaaccacct aaatcaccag ttctgatacc tgcatccaaa acctttttaa
     2701 ctgcatcttc aatggcctta ccttcttcag gcaagttcaa tgacaatttc aacatcattg
     2761 cagcagacaa gatagtggcg atagggttga ccttattctt tggcaaatct ggagcagaac
     2821 cgtggcatgg ttcgtacaaa ccaaatgcgg tgttcttgtc tggcaaagag gccaaggacg
     2881 cagatggcaa caaacccaag gaacctggga taacggaggc ttcatcggag atgatatcac
     2941 caaacatgtt gctggtgatt ataataccat ttaggtgggt tgggttctta actaggatca
     3001 tggcggcaga atcaatcaat tgatgttgaa ccttcaatgt agggaattcg ttcttgatgg
     3061 tttcctccac agtttttctc cataatcttg aagaggccaa aacattagct ttatccaagg
     3121 accaaatagg caatggtggc tcatgttgta gggccatgaa agcggccatt cttgtgattc
     3181 tttgcacttc tggaacggtg tattgttcac tatcccaagc gacaccatca ccatcgtctt
     3241 cctttctctt accaaagtaa atacctccca ctaattctct gacaacaacg aagtcagtac
     3301 ctttagcaaa ttgtggcttg attggagata agtctaaaag agagtcggat gcaaagttac
     3361 atggtcttaa gttggcgtac aattgaagtt ctttacggat ttttagtaaa ccttgttcag
     3421 gtctaacact accggtaccc catttaggac cacccacagc acctaacaaa acggcatcag
     3481 ccttcttgga ggcttccagc gcctcatctg gaagtggaac acctgtagca tcgatagcag
     3541 caccaccaat taaatgattt tcgaaatcga acttgacatt ggaacgaaca tcagaaatag
     3601 ctttaagaac cttaatggct tcggctgtga tttcttgacc aacgtggtca cctggcaaaa
     3661 cgacgatctt cttaggggca gacattagaa tggtatatcc ttgaaatata tatatatatt
     3721 gctgaaatgt aaaaggtaag aaaagttaga aagtaagacg attgctaacc acctattgga
     3781 aaaaacaata ggtccttaaa taatattgtc aacttcaagt attgtgatgc aagcatttag
     3841 tcatgaacgc ttctctattc tatatgaaaa gccggttccg gcctctcacc tttccttttt
     3901 ctcccaattt ttcagttgaa aaaggtatat gcgtcaggcg acctctgaaa ttaacaaaaa
     3961 atttccagtc atcgaatttg attctgtgcg atagcgcccc tgtgtgttct cgttatgttg
     4021 aggaaaaaaa taatggttgc taagagattc gaactcttgc atcttacgat acctgagtat
     4081 tcccacagtt aactgcggtc aagatatttc ttgaatcagg cgccttagac cgctcggcca
     4141 aacaaccaat tacttgttga gaaatagagt ataattatcc tataaatata acgtttttga
     4201 acacacatga acaaggaagt acaggacaat tgattttgaa gagaatgtgg attttgatgt
     4261 aattgttggg attccatttt taataaggca ataatattag gtatgtggat atactagaag
     4321 ttctcctcga tggcgcgccg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4381 accgcatagg gtaataactg atataattaa attgaagctc taatttgtga gtttagtata
     4441 catgcattta cttataatac agttttttag ttttgctggc cgcatcttct caaatatgct
     4501 tcccagcctg cttttctgta acgttcaccc tctaccttag catcccttcc ctttgcaaat
     4561 agtcctcttc caacaataat aatgtcagat cctgtagaga ccacatcatc cacggttcta
     4621 tactgttgac ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt cataatcaac
     4681 caatcgtaac cttcatctct tccacccatg tctctttgag caataaagcc gataacaaaa
     4741 tctttgtcgc tcttcgcaat gtcaacagta cccttagtat attctccagt agatagggag
     4801 cccttgcatg acaattctgc taacatcaaa aggcctctag gttcctttgt tacttcttct
     4861 gccgcctgct tcaaaccgct aacaatacct gggcccacca caccgtgtgc attcgtaatg
     4921 tctgcccatt ctgctattct gtatacaccc gcagagtact gcaatttgac tgtattacca
     4981 atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt acttggcgga taatgccttt
     5041 agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga tatccacatg tgtttttagt
     5101 aaacaaattt tgggacctaa tgcttcaact aactccagta attccttggt ggtacgaaca
     5161 tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga tattaaatag cttggcagca
     5221 acaggactag gatgagtagc agcacgttcc ttatatgtag ctttcgacat gatttatctt
     5281 cgtttcctgc aggtttttgt tctgtgcagt tgggttaaga atactgggca atttcatgtt
     5341 tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct
     5401 tccttctgtt cggagattac cgaatcgcat gcgccatcct catgaaaact gtgtaacata
     5461 ataaccgaag tgtcgaaaag gtggcacctt gtccaattga acacgctcga tgaaaaaaat
     5521 aagatatata taaggttaag taaagcgtct gttagaaagg aagtttttcc tttttcttgc
     5581 tctcttgtct tttcatctac tatttccttc gtgtaataca gggtcgtcag atacatagat
     5641 acaattctat tacccccatc catacaatgc catctcattt cgatactgtt caactacacg
     5701 ccggccaaga gaaccctggt gacaatgctc acagatccag agctgtacca atttacgcca
     5761 ccacttctta tgttttcgaa aactctaagc atggttcgca attgtttggt ctagaagttc
     5821 caggttacgt ctattcccgt ttccaaaacc caaccagtaa tgttttggaa gaaagaattg
     5881 ctgctttaga aggtggtgct gctgctttgg ctgtttcctc cggtcaagcc gctcaaaccc
     5941 ttgccatcca aggtttggca cacactggtg acaacatcgt ttccacttct tacttatacg
     6001 gtggtactta taaccagttc aaaatctcgt tcaaaagatt tggtatcgag gctagatttg
     6061 ttgaaggtga caatccagaa gaattcgaaa aggtctttga tgaaagaacc aaggctgttt
     6121 atttggaaac cattggtaat ccaaagtaca atgttccgga ttttgaaaaa attgttgcaa
     6181 ttgctcacaa acacggtatt ccagttgtcg ttgacaacac atttggtgcc ggtggttact
     6241 tctgtcagcc aattaaatac ggtgctgata ttgtaacaca ttctgctacc aaatggattg
     6301 gtggtcatgg tactactatc ggtggtatta ttgttgactc tggtaagttc ccatggaagg
     6361 actacccaga aaagttccct caattctctc aacctgccga aggatatcac ggtactatct
     6421 acaatgaagc ctacggtaac ttggcataca tcgttcatgt tagaactgaa ctattaagag
     6481 atttgggtcc attgatgaac ccatttgcct ctttcttgct actacaaggt gttgaaacat
     6541 tatctttgag agctgaaaga cacggtgaaa atgcattgaa gttagccaaa tggttagaac
     6601 aatccccata cgtatcttgg gtttcatacc ctggtttagc atctcattct catcatgaaa
     6661 atgctaagaa gtatctatct aacggtttcg gtggtgtctt atctttcggt gtaaaagact
     6721 taccaaatgc cgacaaggaa actgacccat tcaaactttc tggtgctcaa gttgttgaca
     6781 atttaaagct tgcctctaac ttggccaatg ttggtgatgc caagacctta gtcattgctc
     6841 catacttcac tacccacaaa caattaaatg acaaagaaaa gttggcatct ggtgttacca
     6901 aggacttaat tcgtgtctct gttggtatcg aatttattga tgacattatt gcagacttcc
     6961 agcaatcttt tgaaactgtt ttcgctggcc aaaaaccatg agtgtgcgta atgagttgta
     7021 aaattatgta taaacctact ttctctcaca agctcgaggg ggggcccggt acccagcttt
     7081 tgttcccttt agtgagggtt aattccgagc ttggcgtaat catggtcata gctgtttcct
     7141 gtgtgaaatt gttatccgct cacaattcca cacaacatag gagccggaag cataaagtgt
     7201 aaagcctggg gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc
     7261 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7321 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7381 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7441 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7501 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcg gcccccctga cgagcatcac
     7561 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7621 ttcccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7681 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     7741 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7801 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7861 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7921 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     7981 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8041 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8101 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8161 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8221 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8281 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8341 tccatagttg cctgactgcc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8401 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8461 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8521 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8581 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8641 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgaaaa
     8701 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8761 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8821 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8881 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8941 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9001 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9061 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9121 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9181 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9241 ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt ccttttcatc acgtgctata
     9301 aaaataatta taatttaaat tttttaatat aaatatataa attaaaaata gaaagtaaaa
     9361 aaagaaatta aagaaaaaat agtttttgtt ttccgaagat gtaaaagact ctagggggat
     9421 cgccaacaaa tactaccttt tatcttgctc ttcctgctct caggtattaa tgccgaattg
     9481 tttcatcttg tctgtgtaga agaccacaca cgaaaatcct gtgattttac attttactta
     9541 tcgttaatcg aatgtatatc tatttaatct gcttttcttg tctaataaat atatatgtaa
     9601 agtacgcttt ttgttgaaat tttttaaacc tttgtttatt tttttttctt cattccgtaa
     9661 ctcttctacc ttctttattt actttctaaa atccaaatac aaaacataaa aataaataaa
     9721 cacagagtaa attcccaaat tattccatca ttaaaagata cgaggcgcgt gtaagttaca
     9781 ggcaagcgat ccgtcctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     9841 tatcacgagg ccctttcgtc
//
