LOCUS       sequence-51608-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3357 (=HL 716 + pHL 1191)
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     71..89
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    110..128
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    135..153
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(209..237)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     833..853
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
       61 aacctataaa aataggcgta tcacgaggcc ctttcgtctt cacctcgagt ccctatcagt
      121 gatagagatt gacatcccta tcagtgatag agatactgtc gactaaggag gaaaaaaaat
      181 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
      241 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
      301 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
      361 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca
      421 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
      481 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
      541 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
      601 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
      661 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
      721 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
      781 gtccacacaa tctgcccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
      841 ttgagtttgt aacagctgct gggattacac atggcatgga
//
