LOCUS       sequence-51609-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3357 (=HL 716 + pHL 1191)
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    153..169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(221..307)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     460..477
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(563..583)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gcttatcgcc gggctttttt atggcaaaaa aaagcggatc ctggagatcc gcaaaagttc
       61 acgttggctt tagttattcg agttgagaaa ctctcgaaac gggcagtgac ttcaagggtt
      121 aaaagaggtg ccgctccgtt tccgggagta tcttgttatc cgctcacaat gtcaatgtta
      181 tccgctcaca tttatggatc ccctaggtct agggcggcgg atttgtccta ctcaggagag
      241 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      301 ttatttgatg cctggcagtt ccctactctc gcatggggag accccacact accatcggcg
      361 ctacggcgtt tcacttctga gttcggcatg gggtcaggtg ggaccaccgc gctactgccg
      421 ccaggcaaat tctgttttat cagaccgctt ctgcgttctg atttaatctg tatcaggctg
      481 aaaatcttct ctcatccgcc aaaacagcca agctagcttt tatttgtaga gctcatccat
      541 gccatgtgta atcccagcag ctgttacaaa ctcaagaagg accatgtggt ctctcttttc
      601 gttgggatct ttcgaaaggg cagattgtgt ggacaggtaa tggttgtctg gtaaaaggac
      661 agggccatcg ccaattggag tattttgttg ataatggtct gctagttgaa cgcttccatc
      721 ttcaatgttg tgtctaattt tgaagttaac tttgattcca ttcttttgtt tgtctgccat
      781 gatgtataca ttgtgtgagt tatagttgta ttccaatttg tgtccaag
//
