LOCUS       sequence-51610-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Bahler J, Wu JQ, Longtine MS, Shah NG, McKenzie A 3rd, Steever AB, 
            Wach A, Philippsen P, Pringle JR
  TITLE     Heterologous modules for efficient and versatile PCR-based gene 
            targeting in Schizosaccharomyces pombe.
  JOURNAL   Yeast. 1998 Jul;14(10):943-51.
  PUBMED    9717240
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "943-51"
COMMENT     Sequence Label: pFA6a-3HA-kanMX6
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(41..384)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      complement(459..646)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     567..591
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(679..768)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        complement(835..853)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(836..853)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ttaatcgcgg cctcgaaacg tgagtctttt ccttacccat ggttgtttat gttcggatgt
       61 gatgtgagaa ctgtatccta gcaagatttt aaaaggaagt atatgaaaga agaacctcag
      121 tggcaaatcc taacctttta tatttctcta caggggcgcg gcgtggggac aattcaacgc
      181 gtctgtgagg ggagcgtttc cctgctcgca ggtctgcagc gaggagccgt aatttttgct
      241 tcgcgccgtg cggccatcaa aatgtatgga tgcaaatgat tatacatggg gatgtatggg
      301 ctaaatgtac gggcgacagt cacatcatgc ccctgagctg cgcacgtcaa gactgtcaag
      361 gagggtattc tgggcctcca tgtcgctggc cgggtgaccc ggcggggacg aggcaagcta
      421 aacagatcta tattaccctg ttatccctag cggatctgcc ggtagaggtg tggtcaataa
      481 gagcgacctc atactatacc tgagaaagca acctgaccta caggaaagag ttactcaaga
      541 ataagaattt tcgttttaaa acctaagagt cactttaaaa tttgtataca cttatttttt
      601 ttataactta tttaataata aaaatcataa atcataagaa attcgcttat ttagaagtgg
      661 cgcgcctcag cactgagcag cgtaatctgg aacgtcatat ggataggatc ctgcatagtc
      721 cgggacgtca tagggatagc ccgcatagtc aggaacatcg tatgggtaaa agatgttaat
      781 taacccgggg atccgtcgac ctgcagcgta cgaagcttca gctggcggcc gcgttctata
      841 gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc
      901 taacgacaat atgtccatat ggtgcactct cagtacaatc tgctctgat
//
