LOCUS       sequence-51615-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00495
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(176..193)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
       61 ctttcttata atgccaactt tgtacaagaa agttggcaag ttggccaggc gccaagtggt
      121 gggtgcagtc tcggtttgct ttacctgctg ctcctccaca ttgaggtcga tggccatggt
      181 gatggtggtg gtgggcggac tcttgccctc gatcacctcg atggtgaccg attgcggcag
      241 cggcttcaca gctcccttaa ctctcgccgg atcatggctg cgcatgtggg catttagacc
      301 gccggactgg gcgaacgtct tgccgcacaa tttgcacgcg tacttgcgct gctcagagtg
      361 gagcagcttg tgcacccgca gcgccttctt ccgttgaaac gtggagccgc aaacgtcgca
      421 gacgaagggc cgcaaattgg tatgcgatac agcatggaca ttgaggtcat gaaagctata
      481 gaatgccttg ccgcacacct cgcatttggc cggcctttcg cccgtatggc gacgcatatg
      541 cgccgtgaga acgcctcgat ctcggcaagg tcgtccgcac acctcgcaga tgcagggcat
      601 gcgaccagta tggcagatac tgtgcgagga gagctccttt tgcgtgtaaa aggtcgcgtc
      661 gcattccgga cacttgtgcg gcttgatgcc actgtgtcgc tggcggtggc gcatcatgtt
      721 gcccgactgg
//
