LOCUS       sequence-51618-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3375 (=HL 865 + pHL 1008)
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     74..92
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    113..131
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    138..156
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(212..240)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
       61 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcacctcg agtccctatc
      121 agtgatagag attgacatcc ctatcagtga tagagatact gtcgactaag gaggaaaaaa
      181 aatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      241 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      301 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      361 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      421 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      481 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      541 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      601 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      661 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      721 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      781 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc ac
//
