LOCUS       sequence-51622-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00499
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             752..760
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
       61 ctttcttata atgccaactt tgtacaagaa agttggcaat ttgaccgccg cctgtgcctc
      121 cttcttggag ttaaccagct cgaccagctc tttgacgcga cgtaagcagt cctttttact
      181 gcgattcggt atgcaggcgg cgatgcaatc ccagcgatcg ggtgtcgtcg ttggataggt
      241 tttaatggcc tgttcgagca gggcttgctc ctccttggtc caggttttgg aggcagcacc
      301 gccgcccgta gacccatttg tggccggaac tggggctggt gcagccgtgg ggtttgctgg
      361 agctggagcc gtgccattct gcttggttga ctgattgttc gccttatgat ctacaccgtt
      421 ttgtttcaag ttctccttgc tcgcctgggc cgtctcctcg cccagggtta tatccttaca
      481 cgtctgaaca tctttcttgg acttctcgaa actggcaaat gccgcatcgt tggcttgagt
      541 cttcagggag ctcttcgagt gatctgtgtt ctggagcgcc ttggctttgt tgagcacatc
      601 cctggcattg accagaacag tgttatccgg gctatgctgg ttaatgaaag tggctatgac
      661 atcccaacgc tgtgcagtgc cagcgggaaa cagattaacc gccttgataa gcaactgcac
      721 gttctcattg ctccagagct cattcttctt gacctcctta acgccttttg gcgtggccgc
      781 tgagctggca agcttcttgg cctgtgtctg gttaatctcc tccaactcgg cagctatctt
      841 ctgctcggcc gtttgaa
//
