LOCUS       sequence-51623-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3448 (=HL 3425 + pHL 1191)
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    154..170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(222..308)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     461..478
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(564..584)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggcttatcgc cgggcttttt tatggcaaaa aaaagcggat cctggagatc cgcaaaagtt
       61 cacgttggct ttagttattc gagttgagaa actctcgaaa cgggcagtga cttcaagggt
      121 taaaagaggt gccgctccgt ttccgggagt atcttgttat ccgctcacaa tgtcaatgtt
      181 atccgctcac atttatggat cccctaggtc tagggcggcg gatttgtcct actcaggaga
      241 gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt
      301 tttatttgat gcctggcagt tccctactct cgcatgggga gaccccacac taccatcggc
      361 gctacggcgt ttcacttctg agttcggcat ggggtcaggt gggaccaccg cgctactgcc
      421 gccaggcaaa ttctgtttta tcagaccgct tctgcgttct gatttaatct gtatcaggct
      481 gaaaatcttc tctcatccgc caaaacagcc aagctagctt ttatttgtag agctcatcca
      541 tgccatgtgt aatcccagca gctgttacaa actcaagaag gaccatgtgg tctctctttt
      601 cgttgggatc tttcgaaagg gcagattgtg tggacaggta atggttgtct ggtaaaagga
      661 cagggccatc gccaattgga gtattttgtt gataatggtc tgctagttga acgcttccat
      721 cttcaatgtt gtgtctaatt ttgaagttaa ctttgattcc attcttttgt ttgtctgcca
      781 tgatgtatac attgtgtgag ttatagttgt attccaattt gt
//
