LOCUS       sequence-51639-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Knobel PA, Kotov IN, Felley-Bosco E, Stahel RA, Marti TM
  TITLE     Inhibition of REV3 expression induces persistent DNA damage and 
            growth arrest in cancer cells.
  JOURNAL   Neoplasia. 2011 Oct;13(10):961-70.
  PUBMED    22028621
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neoplasia."; date: "2011-10"; volume: "13(10)"; pages: "961-70"
COMMENT     Sequence Label: pSUPERIOR.puro-shREV3-4
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..62
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     47..63
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    129..147
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(193..212)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        358..857
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(393..416)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     790..809
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(882..901)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gaataccctc actaaaggga acaaaagctg gtaccgggcc ccccctcgag gtcgacggta
       61 tcgataagct taaaaacaaa gatgctgcta cattatctct tgaataatgt agcagcatct
      121 ttgggggatc tctatcactg atagggaact tataagattc ccaaatccaa agacatttca
      181 cgtttatggt gatttcccag aacacatagc gacatgcaaa tattgcaggg cgccactccc
      241 ctgtccctca cagccatctt cctgccaggg cgcacgcgcg ctgggtgttc ccgcctagtg
      301 acactgggcc cgcgattcct tggagcgggt tgatgacgtc agcgttcgaa ttctaccggg
      361 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
      421 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
      481 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
      541 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
      601 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
      661 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
      721 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
      781 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
      841 catctccggg cctttcgacc tgcagcccaa gctagcttac catgaccgag tacaagccca
      901 cggtgcgcct cgccacccgc gacgacgtcc cc
//
