LOCUS       sequence-51640-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Knobel PA, Kotov IN, Felley-Bosco E, Stahel RA, Marti TM
  TITLE     Inhibition of REV3 expression induces persistent DNA damage and 
            growth arrest in cancer cells.
  JOURNAL   Neoplasia. 2011 Oct;13(10):961-70.
  PUBMED    22028621
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neoplasia."; date: "2011-10"; volume: "13(10)"; pages: "961-70"
COMMENT     Sequence Label: pSUPERIOR.puro-shREV3-5
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..60
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     45..61
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    127..145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(191..210)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        356..855
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(391..414)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     788..807
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(880..899)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 taccctcact aaagggaaac aaaagctggt accgggcccc ccctcgaggt cgacggtatc
       61 gataagctta aaaagatatt ccatctgtta caatctcttg aattgtaaca gatggaatat
      121 cggggatctc tatcactgat agggaactta taagattccc aaatccaaag acatttcacg
      181 tttatggtga tttcccagaa cacatagcga catgcaaata ttgcagggcg ccactcccct
      241 gtccctcaca gccatcttcc tgccagggcg cacgcgcgct gggtgttccc gcctagtgac
      301 actgggcccg cgattccttg gagcgggttg atgacgtcag cgttcgaatt ctaccgggta
      361 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
      421 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
      481 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
      541 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
      601 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
      661 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
      721 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
      781 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
      841 tctccgggcc tttcgacctg cagcccaagc tagcttacca tgaccgagta caagcccacg
      901 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgcc
//
