LOCUS       sequence-51668-e       13709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13709)
  AUTHORS   Andersson CR, Tsinoremas NF, Shelton J, Lebedeva NV, Yarrow J, Min 
            H, Golden SS
  TITLE     Application of bioluminescence to the study of circadian rhythms in 
            cyanobacteria.
  JOURNAL   Methods Enzymol. 2000;305:527-42.
  PUBMED    10812624
REFERENCE   2  (bases 1 to 13709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2000"; volume: "305"; pages: "527-42"
COMMENT     Sequence Label: pAM1706
FEATURES             Location/Qualifiers
     source          1..13709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2215..3327)
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(3973..4896)
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             complement(4908..6350)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     terminator      complement(6863..6890)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6982..7068)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        complement(7647..7751)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7954..8056
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             8057..8716
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8123..8142)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(9780..9799)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             11261..11452
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(11446..11468)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11554..11694
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11609..11628
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11709..11726)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11880..12468)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11960..11979)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12639..13430,13431..13499))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13500..13604)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agcttgtcat ctgccggatg aggcaaaacc ctgcctacgg cgcgattaca tcgtcccagc
       61 gcgatcgctc ttactgttga tggctcgtgc ttaaaaacaa tgcaaacttc accgtttcag
      121 ctggtgattt tcgactgtga tggtgtgctt gttgatagcg gaacgcatca ctaatcgcgt
      181 ctttgcagac atgctcaatg aactgggtct gttggtgact ttggatgaca tgtttgagca
      241 gtttgtgggt cattccatgg ctgactgtct caaactaatt gagcgacggt taggcaatcc
      301 tccaccccct gactttgttc agcactatca acgccgtacc cgtatcgcgt tagaaacgca
      361 tctacaagcc gttcctgggg ttgaagaggc tttggatgct cttgaattgc cctactgtgt
      421 tgcgtccagt ggtgatcatc aaaagatgcg aaccacactg agcctgacga agctctggcc
      481 acgatttgag ggacgaatct tcagcgtgac tgaagtacct cgcggcaagc catttcccga
      541 tgtctttttg ttggccgccg atcgcttcgg ggttaatcct acggcctgcg ctgtgatcga
      601 agacaccccc ttgggagtag cggcaggcgt ggcggcagga atgcaagtgt ttggctacgc
      661 gggttccatg cccgcttggc gtctgcaaga agccggtgcc catctcattt ttgacgatat
      721 gcgactgctg cccagtctgc tccaatcgtc gccaaaagat aactccacag cattgcccaa
      781 tccctaaccc ctgctcgcgc cgcaactaca cactaaaccg ttcctgcgcg atcgctctta
      841 ctgttgatgg ctcgtgctta aaaacaatgc aaccctaacc gtttcagctg gtgattttcg
      901 gacgatttgg cttacaggga taactgagag tcaacagcct ctgtccgtca ttgcacaccc
      961 atccatgcac tggggacttg actcatgctg aatcacattt cccttgtcca ttgggcgaga
     1021 ggggagggga atcttctgga ctcttcacta agcggcgatc gcaggttctt ctacccaagc
     1081 agtggcgatc gcttgattgc agtcttcaat gctggcctct gcagccatcg ccgccaccaa
     1141 agcatcgtag gcgggacgtt gttgctccag taaagtcttc gcccgtaaca atccccagcg
     1201 actgcgtaaa tccgcttcgg caggattgcg atcgagttgc cgccacagtt gtttccactg
     1261 ggcgcgatcg tcagctcccc cttccacgtt gccgtagacc agttgctctg ccgctgcacc
     1321 ggccatcaac acctgacacc actgttccag cgatcgctga ctgagttgcc cctgtgcggc
     1381 ttcggcttct agcgcagctg cttggaactg cacacccccg cgaccaggtt gtccttggcg
     1441 cagcgcttcc cacgctgaga gggtgtagcc cgtcacgggt aaccgatggg taccgagctc
     1501 gaattgaatt gaattccttt aatcccttta attcctggat tttttctccg aaatctggga
     1561 gcgtataaac atctggactc gtataaatca gttgactggt ttctatgcct tctttgccac
     1621 atattatatg tatatcaact aataccgtat tattatcata acctatagac atagttccat
     1681 tgtattttag cgcgttctca aattcttctt ttgatggata cgatgatcca tctccgtact
     1741 ttttcgttaa cgatttgctt actggcttca caagtctgcc aatattaatc tctaatcttt
     1801 taaatttccc atttaaaaaa aatgccattg cttcacgctt tttgttagca aacgaaaagt
     1861 cagaacatga ataaagtgat acttcttcta ttcgagaatc tccctcaaat ttttcaaaat
     1921 cacataacct cttcgaaaga aactcttctt ctgtcatgcc aaactttgca tctttatacc
     1981 catcaactgc cattgcacca aagctaatca ttcccaatgc aagcataact gccaattttt
     2041 tcatgttatc tccttttgct ttgagccaat aataacaatg agaacaatgt taagccaacc
     2101 ccataaacct ctatcaagta cccaaatgtg gatgttttgg agaattttgt aaagtgtaga
     2161 tgtcattcag atttatataa cgcaagcatt ccacttacaa ttaggcaaag gatatcaact
     2221 atcaaacgct tcggttaagc ttaaagcaca ccctttctgc gtcctcgtat tgacgcgacg
     2281 taaaatttca acgagcacgc cgggatactt accatattct ctgctaatta tcccgacatc
     2341 atcggtaaca ataaatgctg gataactggt tgctgacgca tccatataac tcatcaaccc
     2401 cggcgttcca tcaggtacag gtttcaacgt ttcaggatca agcgctcgcg catataccca
     2461 cggcggaaca tgtttacgct gcatttcatc ctcaaagaaa caagtgttga gttcaacttg
     2521 attaaatata tctcggatct gactaatatc actgagattg aaagtatcaa ataaaagatg
     2581 attgaaatca tcacgtttca gagattcttt ttcgtaactt ttccagccgc ctccggttat
     2641 gatataaagg cttttatctc cagaaaatga gattttttta tctttcatat aatggcagag
     2701 taaataaata aagtatggcg aaccaataag acaaagatct ttcccttgat tttttattcg
     2761 ttcaagacta ttcaatgttt taacaaaatc tattcgttct tctgttacgg taaatgtcgt
     2821 aggatataac aattccacca aactcataac atatttaaac caaatattat gagcattaaa
     2881 tctatctggt cccaaattga ctaattctat ttgatgatca aaccaactac caacatattt
     2941 catgccataa ctcacagagc ctaagagtct ctcaatactt aatctgtcac gcgccacctg
     3001 actttttaaa ccattcgtgc cgctactggt aaaccaactt tcaatctcgt tttcctgaga
     3061 agttaataag cgagtaaact taaaaaccga tgttgggaat acaggtatgt catcaatttc
     3121 cgtaatattg tcatctactt tgtgtgcctg acagtagtga cgatattctc gacaatgttt
     3181 ataatgatta cgaaatgcat caagcacaag tttctttctg attttttcct gctcgtcgta
     3241 agaccacact aatggatcgc tcgaaaaaat caaatcatca atttctgagc ttgctgtaat
     3301 ttcttgttta tcaacatatg aagtcatacc tgttttactc ctcaagataa tattagaaag
     3361 tatggcagca ctgctgtcat actcttttat acccttcatc tttcaagctg ctgctttgtt
     3421 ggctgctttc actcacccca gtcacatagt tatctatgct cctggggatt cgttcacttg
     3481 ccgcctcgct gcaactcgaa atctattagg tatattccat gtggtacttc ttaatattat
     3541 catcaacaat attgattaca tttttttggc tcatcaaatc attcattggt tcaaaggaca
     3601 gcaatacact tttcgcacca cacttttcaa ttgccaactt agccgcagtt atacactccg
     3661 tataatttcc gacagcgttt tctgcaatta tttcttcaag tttattttcg aaattttcat
     3721 tagggtgcat ttcaagaaca taatcactaa taaatgcacg cggttaccaa gcaaaccaaa
     3781 ctccgtgaaa tgatgctcca gtaaccatac ggtatcaaaa ccacactcct cagagatgcg
     3841 acctaattta accaaacgtt tcattacctc tgtttgagaa aattggggag gttggtatgt
     3901 aagcaaaaag tttccaaatt tcatagagag tccttatatt gctatttgag tgatagaata
     3961 tctcaataga ttttaagaca gagaaattgc ttgattttca atctcaattc tcattcggcg
     4021 ttcattgact gtcgcaatag ttaaatgttc aaatgacggt tcagtaatat caacatcaat
     4081 atccagatga tcattatcca tcgcgatagc ggctttcgta accgattgat aaaaattgcg
     4141 caggaccact aaattttcac tcaagtcatg cgaacttcct aacaaagaat atatcttgca
     4201 tcgattacta cgaatatttg ataacaatgt gataacttca tcttgcttga cccaattatc
     4261 gttatttgca gtaaaagcaa taaacggtat atcaagatac atcatgttat taattgtaga
     4321 agctaaatct tcccaaccaa aatcaagaca atctctcgca aagacttcag cacccaattt
     4381 atggccttca aaatctagat tatccggcaa ttcattaatg ggtagactga gataatcaaa
     4441 ccctaaagct ctttcaagag aatatcttaa gttaacaaca ccgactgcgg tgattaaaaa
     4501 cgaagcattg atttcagata ggcttgcata agctatccgc gcagataagc ttgaagccaa
     4561 cataccgaag ttatttattt ttcgtgtagt taaccaatca accactgcta acaagctctg
     4621 ctttcctata gacattgtaa attcatcaat tgtccctgaa ctcaatccaa cgtggtgaag
     4681 cgaatcatag cggatcacat gaaatccatt ccgcgataaa tattccgcca gaccagcaaa
     4741 atgatccatc ctgcgggcaa aaccagacgc aataataatg gcattctttc tctttgggct
     4801 gttttcttct ggcagcgttt cccaaacatg aattttttta tttccttcaa cacaaataac
     4861 gtggtcgatg gttttatatt ttgattcatt ttccatactt ttacctatta tgggacaaat
     4921 acaaggaact tatcttcttc caggaatcga gtctgttcta tttcaaccgc aacatcctta
     4981 gccgtatagt tagatggcct ttcatgagaa atatatgtca ctaatcgttg caacggtctc
     5041 attccgtcat gagatccacc aactcgaaat atgttattca ttcctgcttc tacaatcctt
     5101 tccgcacctt ttaatgctaa cgcatctcga tatttaaatg atgactccca aggaaaaata
     5161 gatatggttt gcgtcttatt tttttgaaca taaggcaata tttgctcaat attatcgacg
     5221 tgatgaaggt acacacatct gccaagtggt tgattaaatt ccacacctgc atttgactca
     5281 ataatcatcc aacgttgatg aatatccacc tctactttta atccagcaaa caagctttct
     5341 ttttgaacta aagaataggc cgccttttca tcaaaatctt ttttggcatt cggtaatata
     5401 tgcgcatata gattaagttt ttctatcaac gctaacttaa attcctcata atgatttccc
     5461 atgtaatata tgttttgggc agaaaaacaa gctcgctgat cgtaaaaaca aacatcatga
     5521 gccgcacctg tcgctgcgga cgtcaaatca acaggattat cgataatgca aagactcttt
     5581 ttagaaccaa atttaatcac atcagcataa gatggcgcat gctctaccgc ccaattaatc
     5641 gcatctggcc ctccccaagc gacaataaca tccgcatgtc gcataatttc ttttgcgagt
     5701 gatgtatcac cttggtgggg ccaatatata acagataaag agcgcgttat cggatgatta
     5761 gggtctacat caataaaact taacgctaat gcattagcgg taaaaggatc ggttgacgat
     5821 gtttttataa tacactgatt cttagttaaa attgcgcgta atatagacat gatcccagat
     5881 aatggaacat tacctgccaa cagatgtaca gatttacctt tcggaaaagc ccgaacataa
     5941 ctttcatcct gaggtagcca ttcatccatg atatggcgag aaccaagttc attttctaca
     6001 acatcataaa ggccgccttt agaacataaa atcatagata tccaattggc ctctagctta
     6061 gccatttctt ctgaatatcc catatatttt tttaagtcac gaatgtatgt cctgcgtctt
     6121 gagtattctt catttttcca tctttgccct accgtataga gaaaattgac aatgttatgc
     6181 aaccgtaatt cgttatttcc attacaatca ataatgtttt ttacatgaga gtcattcaat
     6241 attggcaggt aaacactatt atcaccaaaa ttaatggatt gcactaaatc atcactttcg
     6301 ggaaagattt caacctggcc gttaataatg aatgaaattt ttttagtcat atttgccatc
     6361 cacgagctcg gtacccatcg tcgacgggag gggctgcaat gaacgcaacg atgaagcaga
     6421 tggtggcggt cagcagagtg gggatcatca gcacgccgaa ccaacccacg tagatgcggt
     6481 tgtcggtgct ggttacccac tcacaaaacc gatcccaaac gttatcgcgg cgttgctcgc
     6541 gaagaatgct ggtcatatcg atcttgaggt tgtaaagggc aagagtctta gttaaaaact
     6601 cttgcttttt aggctaggag attaactgtc aaaaaggtgt aaaaaaccct taatttttag
     6661 ctaagtagac actattttta tgtaacgaaa actttgtgaa tttatgtaat gtttagagcg
     6721 atcgcccaag gctacagtgc tcaacggagc caaaactact gtctacactg gattagaaga
     6781 cgctaaatcc agcctctaga cccgggctcg agctagcaag cttggccgga tccggccgga
     6841 tccgggagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttaatttg
     6901 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac
     6961 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     7021 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggcagt
     7081 tccctactct cgcatgggga gaccccacac taccatcggc gctacggcgt ttcacttctg
     7141 agttcggcat ggggtcaggt gggaccaccg cgctactgcc gccaggcaaa ttctgtttta
     7201 ttgagccgtt accccaccta ctagctaatc ccatctgggc acatccgatg gcaagaggcc
     7261 cgaaggtccc cctctttggt cttgcgacgt tatgcggtat tagctaccgt ttccagtagt
     7321 tatccccctc catcaggcag tttcccagac attactcacc cgtccgccac tcgtcagcaa
     7381 agaagcaagc ttagatccgt cacacgggat aataccgcgc cacatagcag aactttaaaa
     7441 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7501 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7561 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7621 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7681 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7741 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     7801 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttcg aataaatacc
     7861 tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg ataccgggaa
     7921 gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag gttccaactt
     7981 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
     8041 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     8101 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     8161 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     8221 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     8281 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     8341 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     8401 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     8461 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     8521 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     8581 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     8641 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     8701 gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggttgcta
     8761 cgcctgaata agtgataata agcggatgaa tggcagaaat tcgaaagcaa attcgacccg
     8821 gtcgtcggtt cagggcaggg tcgttaaata gccgcttatg tctattgctg gtttaccggt
     8881 ttattgacta ccggaagcag tgtgaccgtg tgcttctcaa atgcctgagg ccagtttgct
     8941 caggctctcc ccgtggaggt aataattgac gatatgatcg acggatctgg taaccccagc
     9001 gcggttgcta ccaagtagtg acccgcttcg tgatgcaaaa tccgctgacg atattcgggc
     9061 gatcgctgct gaatgccatc gagcagtaac gtggcacccc gcccctgcca agtcaccgca
     9121 tccagactga acagcaccaa gaggctaaaa cccaatcccg ccggtagcag cggagaacta
     9181 cccagcattg gtcccaccaa agctaatgcc gtcgtggtaa aaatcgcgat cgccgtcaga
     9241 ctcaagccca gttcgctcat gcttcctcat ctaggtcaca gtcttcggcg atcgcatcga
     9301 tctgatgctg cagcaagcgt tttccatacc ggcgatcgcg ccgtcgccct ttcgctgccg
     9361 tggcccgctt acgagctcgt ttatcgacca cgatcgcatc caaatccgcg atcgcttccc
     9421 agtccggcaa ttcagtctgg ggcgtccgtt tcattaatcc tgatcaggca cgaaattgct
     9481 gtgcgtagta tcgcgcatag cggccagcct ctgccaacag cgcatcgtga ttgcctgcct
     9541 caacaatctg gccgcgctcc atcaccaaga tgcggctggc attacgaacc gtagccagac
     9601 ggtgagcaat gataaagacc gtccgtccct gcatcacccg ttctagggcc tcttgcacca
     9661 aggtttcgga ctcggaatca agcgccgaag tcgcctcatc cagaattaaa atgcgtggat
     9721 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     9781 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     9841 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     9901 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     9961 cttcctaatg caggagtcgc ataagggaga gcgtcgatcg accgatgccc ttgagagcct
    10021 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
    10081 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
    10141 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
    10201 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
    10261 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
    10321 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
    10381 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
    10441 aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg
    10501 cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc
    10561 tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct
    10621 gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca
    10681 attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc
    10741 cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt
    10801 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg
    10861 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc
    10921 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac
    10981 gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct
    11041 accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt
    11101 tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg
    11161 ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat
    11221 tacccccatg aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac
    11281 cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa
    11341 cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga
    11401 gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    11461 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    11521 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
    11581 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
    11641 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
    11701 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    11761 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    11821 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    11881 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    11941 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    12001 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    12061 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    12121 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    12181 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    12241 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    12301 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
    12361 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    12421 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    12481 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    12541 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    12601 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    12661 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    12721 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    12781 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    12841 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    12901 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
    12961 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    13021 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    13081 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    13141 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    13201 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
    13261 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    13321 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    13381 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    13441 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    13501 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    13561 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    13621 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    13681 atcacgaggc cctttcgtct tcaagaatt
//
