LOCUS       sequence-51670-e       11586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Mackey SR, Ditty JL, Clerico EM, Golden SS
  TITLE     Detection of rhythmic bioluminescence from luciferase reporters in 
            cyanobacteria.
  JOURNAL   Methods Mol Biol. 2007;362:115-29.
  PUBMED    17417005
REFERENCE   2  (bases 1 to 11586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2007"; volume: "362"; pages: "115-29"
COMMENT     Sequence Label: pAM1619
FEATURES             Location/Qualifiers
     source          1..11586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2210..3322)
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(3968..4891)
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             complement(4904..6346)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     primer_bind     complement(7657..7676)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             9138..9329
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(9323..9345)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9431..9571
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9486..9505
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9586..9603)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9757..10345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9837..9856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10516..11307,11308..11376))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11139..11158
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11377..11481)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11549..11567
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agcttgtcat ctgccggatg aggcaaaacc ctgcctacgg cgcgattaca tcgtcccagc
       61 gcgatcgctc ttactgttga tggctcgtgc ttaaaaacaa tgcaaacttc accgtttcag
      121 ctggtgattt tcgactgtga tggtgtgctt gttgatagcg gaacgcatca ctaatcgcgt
      181 ctttgcagac atgctcaatg aactgggtct gttggtgact ttggatgaca tgtttgagca
      241 gtttgtgggt cattccatgg ctgactgtct caaactaatt gagcgacggt taggcaatcc
      301 tccaccccct gactttgttc agcactatca acgccgtacc cgtatcgcgt tagaaacgca
      361 tctacaagcc gttcctgggg ttgaagaggc tttggatgct cttgaattgc cctactgtgt
      421 tgcgtccagt ggtgatcatc aaaagatgcg aaccacactg agcctgacga agctctggcc
      481 acgatttgag ggacgaatct tcagcgtgac tgaagtacct cgcggcaagc catttcccga
      541 tgtctttttg ttggccgccg atcgcttcgg ggttaatcct acggcctgcg ctgtgatcga
      601 agacaccccc ttgggagtag cggcaggcgt ggcggcagga atgcaagtgt ttggctacgc
      661 gggttccatg cccgcttggc gtctgcaaga agccggtgcc catctcattt ttgacgatat
      721 gcgactgctg cccagtctgc tccaatcgtc gccaaaagat aactccacag cattgcccaa
      781 tccctaaccc ctgctcgcgc cgcaactaca cactaaaccg ttcctgcgcg atcgctctta
      841 ctgttgatgg ctcgtgctta aaaacaatgc aaccctaacc gtttcagctg gtgattttcg
      901 gacgatttgg cttacaggga taactgagag tcaacagcct ctgtccgtca ttgcacaccc
      961 atccatgcac tggggacttg actcatgctg aatcacattt cccttgtcca ttgggcgaga
     1021 ggggagggga atcttctgga ctcttcacta agcggcgatc gcaggttctt ctacccaagc
     1081 agtggcgatc gcttgattgc agtcttcaat gctggcctct gcagccatcg ccgccaccaa
     1141 agcatcgtag gcgggacgtt gttgctccag taaagtcttc gcccgtaaca atccccagcg
     1201 actgcgtaaa tccgcttcgg caggattgcg atcgagttgc cgccacagtt gtttccactg
     1261 ggcgcgatcg tcagctcccc cttccacgtt gccgtagacc agttgctctg ccgctgcacc
     1321 ggccatcaac acctgacacc actgttccag cgatcgctga ctgagttgcc cctgtgcggc
     1381 ttcggcttct agcgcagctg cttggaactg cacacccccg cgaccaggtt gtccttggcg
     1441 cagcgcttcc cacgctgaga gggtgtagcc cgtcacgggt aaccgatggg taccgagctc
     1501 gaattgaatt cctttaatcc ctttaattcc tggatttttt ctccgaaatc tgggagcgta
     1561 taaacatctg gactcgtata aatcagttga ctggtttcta tgccttcttt gccacatatt
     1621 atatgtatat caactaatac cgtattatta tcataaccta tagacatagt tccattgtat
     1681 tttagcgcgt tctcaaattc ttcttttgat ggatacgatg atccatctcc gtactttttc
     1741 gttaacgatt tgcttactgg cttcacaagt ctgccaatat taatctctaa tcttttaaat
     1801 ttcccattta aaaaaaatgc cattgcttca cgctttttgt tagcaaacga aaagtcagaa
     1861 catgaataaa gtgatacttc ttctattcga gaatctccct caaatttttc aaaatcacat
     1921 aacctcttcg aaagaaactc ttcttctgtc atgccaaact ttgcatcttt atacccatca
     1981 actgccattg caccaaagct aatcattccc aatgcaagca taactgccaa ttttttcatg
     2041 ttatctcctt ttgctttgag ccaataataa caatgagaac aatgttaagc caaccccata
     2101 aacctctatc aagtacccaa atgtggatgt tttggagaat tttgtaaagt gtagatgtca
     2161 ttcagattta tataacgcaa gcattccact tacaattagg caaaggatat caactatcaa
     2221 acgcttcggt taagcttaaa gcacaccctt tctgcgtcct cgtattgacg cgacgtaaaa
     2281 tttcaacgag cacgccggga tacttaccat attctctgct aattatcccg acatcatcgg
     2341 taacaataaa tgctggataa ctggttgctg acgcatccat ataactcatc aaccccggcg
     2401 ttccatcagg tacaggtttc aacgtttcag gatcaagcgc tcgcgcatat acccacggcg
     2461 gaacatgttt acgctgcatt tcatcctcaa agaaacaagt gttgagttca acttgattaa
     2521 atatatctcg gatctgacta atatcactga gattgaaagt atcaaataaa agatgattga
     2581 aatcatcacg tttcagagat tctttttcgt aacttttcca gccgcctccg gttatgatat
     2641 aaaggctttt atctccagaa aatgagattt ttttatcttt catataatgg cagagtaaat
     2701 aaataaagta tggcgaacca ataagacaaa gatctttccc ttgatttttt attcgttcaa
     2761 gactattcaa tgttttaaca aaatctattc gttcttctgt tacggtaaat gtcgtaggat
     2821 ataacaattc caccaaactc ataacatatt taaaccaaat attatgagca ttaaatctat
     2881 ctggtcccaa attgactaat tctatttgat gatcaaacca actaccaaca tatttcatgc
     2941 cataactcac agagcctaag agtctctcaa tacttaatct gtcacgcgcc acctgacttt
     3001 ttaaaccatt cgtgccgcta ctggtaaacc aactttcaat ctcgttttcc tgagaagtta
     3061 ataagcgagt aaacttaaaa accgatgttg ggaatacagg tatgtcatca atttccgtaa
     3121 tattgtcatc tactttgtgt gcctgacagt agtgacgata ttctcgacaa tgtttataat
     3181 gattacgaaa tgcatcaagc acaagtttct ttctgatttt ttcctgctcg tcgtaagacc
     3241 acactaatgg atcgctcgaa aaaatcaaat catcaatttc tgagcttgct gtaatttctt
     3301 gtttatcaac atatgaagtc atacctgttt tactcctcaa gataatatta gaaagtatgg
     3361 cagcactgct gtcatactct tttataccct tcatctttca agctgctgct ttgttggctg
     3421 ctttcactca ccccagtcac atagttatct atgctcctgg ggattcgttc acttgccgcc
     3481 tcgctgcaac tcgaaatcta ttaggtatat tccatgtggt acttcttaat attatcatca
     3541 acaatattga ttacattttt ttggctcatc aaatcattca ttggttcaaa ggacagcaat
     3601 acacttttcg caccacactt ttcaattgcc aacttagccg cagttataca ctccgtataa
     3661 tttccgacag cgttttctgc aattatttct tcaagtttat tttcgaaatt ttcattaggg
     3721 tgcatttcaa gaacataatc actaataaat gcacgcggtt accaagcaaa ccaaactccg
     3781 tgaaatgatg ctccagtaac catacggtat caaaaccaca ctcctcagag atgcgaccta
     3841 atttaaccaa acgtttcatt acctctgttt gagaaaattg gggaggttgg tatgtaagca
     3901 aaaagtttcc aaatttcata gagagtcctt atattgctat ttgagtgata gaatatctca
     3961 atagatttta agacagagaa attgcttgat tttcaatctc aattctcatt cggcgttcat
     4021 tgactgtcgc aatagttaaa tgttcaaatg acggttcagt aatatcaaca tcaatatcca
     4081 gatgatcatt atccatcgcg atagcggctt tcgtaaccga ttgataaaaa ttgcgcagga
     4141 ccactaaatt ttcactcaag tcatgcgaac ttcctaacaa agaatatatc ttgcatcgat
     4201 tactacgaat atttgataac aatgtgataa cttcatcttg cttgacccaa ttatcgttat
     4261 ttgcagtaaa agcaataaac ggtatatcaa gatacatcat gttattaatt gtagaagcta
     4321 aatcttccca accaaaatca agacaatctc tcgcaaagac ttcagcaccc aatttatggc
     4381 cttcaaaatc tagattatcc ggcaattcat taatgggtag actgagataa tcaaacccta
     4441 aagctctttc aagagaatat cttaagttaa caacaccgac tgcggtgatt aaaaacgaag
     4501 cattgatttc agataggctt gcataagcta tccgcgcaga taagcttgaa gccaacatac
     4561 cgaagttatt tatttttcgt gtagttaacc aatcaaccac tgctaacaag ctctgctttc
     4621 ctatagacat tgtaaattca tcaattgtcc ctgaactcaa tccaacgtgg tgaagcgaat
     4681 catagcggat cacatgaaat ccattccgcg ataaatattc cgccagacca gcaaaatgat
     4741 ccatcctgcg ggcaaaacca gacgcaataa taatggcatt ctttctcttt gggctgtttt
     4801 cttctggcag cgtttcccaa acatgaattt ttttatttcc ttcaacacaa ataacgtggt
     4861 cgatggtttt atattttgat tcattttcca tactttttac ctattatggg acaaatacaa
     4921 ggaacttatc ttcttccagg aatcgagtct gttctatttc aaccgcaaca tccttagccg
     4981 tatagttaga tggcctttca tgagaaatat atgtcactaa tcgttgcaac ggtctcattc
     5041 cgtcatgaga tccaccaact cgaaatatgt tattcattcc tgcttctaca atcctttccg
     5101 caccttttaa tgctaacgca tctcgatatt taaatgatga ctcccaagga aaaatagata
     5161 tggtttgcgt cttatttttt tgaacataag gcaatatttg ctcaatatta tcgacgtgat
     5221 gaaggtacac acatctgcca agtggttgat taaattccac acctgcattt gactcaataa
     5281 tcatccaacg ttgatgaata tccacctcta cttttaatcc agcaaacaag ctttcttttt
     5341 gaactaaaga ataggccgcc ttttcatcaa aatctttttt ggcattcggt aatatatgcg
     5401 catatagatt aagtttttct atcaacgcta acttaaattc ctcataatga tttcccatgt
     5461 aatatatgtt ttgggcagaa aaacaagctc gctgatcgta aaaacaaaca tcatgagccg
     5521 cacctgtcgc tgcggacgtc aaatcaacag gattatcgat aatgcaaaga ctctttttag
     5581 aaccaaattt aatcacatca gcataagatg gcgcatgctc taccgcccaa ttaatcgcat
     5641 ctggccctcc ccaagcgaca ataacatccg catgtcgcat aatttctttt gcgagtgatg
     5701 tatcaccttg gtggggccaa tatataacag ataaagagcg cgttatcgga tgattagggt
     5761 ctacatcaat aaaacttaac gctaatgcat tagcggtaaa aggatcggtt gacgatgttt
     5821 ttataataca ctgattctta gttaaaattg cgcgtaatat agacatgatc ccagataatg
     5881 gaacattacc tgccaacaga tgtacagatt tacctttcgg aaaagcccga acataacttt
     5941 catcctgagg tagccattca tccatgatat ggcgagaacc aagttcattt tctacaacat
     6001 cataaaggcc gcctttagaa cataaaatca tagatatcca attggcctct agcttagcca
     6061 tttcttctga atatcccata tattttttta agtcacgaat gtatgtcctg cgtcttgagt
     6121 attcttcatt tttccatctt tgccctaccg tatagagaaa attgacaatg ttatgcaacc
     6181 gtaattcgtt atttccatta caatcaataa tgttttttac atgagagtca ttcaatattg
     6241 gcaggtaaac actattatca ccaaaattaa tggattgcac taaatcatca ctttcgggaa
     6301 agatttcaac ctggccgtta ataatgaatg aaattttttt agtcatattt gccatccacg
     6361 agctcggtac ccatcgtcga cgtcgacggg aggggctgca atgaacgcaa cgatgaagca
     6421 gatggtggcg gtcagcagag tggggatcat cagcacgccg aaccaaccca cgtagatgcg
     6481 gttgtcggtg ctggttaccc actcacaaaa ccgatcccaa acgttatcgc ggcgttgctc
     6541 gcgaagaatg ctggtcatat cgatcttgag gttgtaaagg gcaagagtct tagttaaaaa
     6601 ctcttgcttt ttaggctagg agattaactg tcaaaaaggt gtaaaaaacc cttaattttt
     6661 agctaagtag acactatttt tatgtaacga aaactttgtg aatttatgta atgtttagag
     6721 cgatcgccca aggctacagt gctcaacgga gccaaaacta ctgtctacac tggattagaa
     6781 gacgctaaat ccagcctggt atgagtcagc aacaccttct tcacgaggca gacctcagcg
     6841 cccccccccc cctgcaggtc gatctggtaa ccccagcgcg gttgctacca agtagtgacc
     6901 cgcttcgtga tgcaaaatcc gctgacgata ttcgggcgat cgctgctgaa tgccatcgag
     6961 cagtaacgtg gcaccccgcc cctgccaagt caccgcatcc agactgaaca gcaccaagag
     7021 gctaaaaccc aatcccgccg gtagcagcgg agaactaccc agcattggtc ccaccaaagc
     7081 taatgccgtc gtggtaaaaa tcgcgatcgc cgtcagactc aagcccagtt cgctcatgct
     7141 tcctcatcta ggtcacagtc ttcggcgatc gcatcgatct gatgctgcag caagcgtttt
     7201 ccataccggc gatcgcgccg tcgccctttc gctgccgtgg cccgcttacg agctcgttta
     7261 tcgaccacga tcgcatccaa atccgcgatc gcttcccagt ccggcaattc agtctggggc
     7321 gtccgtttca ttaatcctga tcaggcacga aattgctgtg cgtagtatcg cgcatagcgg
     7381 ccagcctctg ccaacagcgc atcgtgattg cctgcctcaa caatctggcc gcgctccatc
     7441 accaagatgc ggctggcatt acgaaccgta gccagacggt gagcaatgat aaagaccgtc
     7501 cgtccctgca tcacccgttc tagggcctct tgcaccaagg tttcggactc ggaatcaagc
     7561 gccgaagtcg cctcatccag aattaaaatg cgtggatcct ctacgccgga cgcatcgtgg
     7621 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     7681 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     7741 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     7801 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     7861 agggagagcg tcgatcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     7921 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     7981 gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc
     8041 gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc
     8101 ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg
     8161 gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc
     8221 cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg
     8281 tccaggcagg tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc
     8341 agcctaactt cgatcactgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc
     8401 acatggaacg ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg
     8461 ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg
     8521 ctaacggatt caccactcca agaattggag ccaatcaatt cttgcggaga actgtgaatg
     8581 cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc agccgcacgc
     8641 ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt
     8701 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
     8761 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
     8821 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca
     8881 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg
     8941 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata
     9001 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc
     9061 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc
     9121 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
     9181 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
     9241 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc
     9301 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     9361 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     9421 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     9481 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     9541 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     9601 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    10141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    10201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10501 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10561 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10621 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10681 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10741 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10801 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
    10861 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10921 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10981 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    11041 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    11101 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
    11161 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    11221 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    11281 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    11341 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    11401 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11461 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    11521 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
    11581 agaatt
//
