LOCUS       sequence-51671-e       16617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16617)
  AUTHORS   Mackey SR, Ditty JL, Clerico EM, Golden SS
  TITLE     Detection of rhythmic bioluminescence from luciferase reporters in 
            cyanobacteria.
  JOURNAL   Methods Mol Biol. 2007;362:115-29.
  PUBMED    17417005
REFERENCE   2  (bases 1 to 16617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2007"; volume: "362"; pages: "115-29"
COMMENT     Sequence Label: pAM2195
FEATURES             Location/Qualifiers
     source          1..16617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(5118..6230)
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(6876..7799)
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIYVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             complement(7812..9254)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     terminator      complement(9767..9794)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(9886..9972)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        complement(10555..10659)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10862..10964
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             10965..11624
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(11031..11050)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(12688..12707)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             14169..14360
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(14354..14376)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    14462..14602
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     14517..14536
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14617..14634)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14788..15376)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14868..14887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15547..16338,16339..16407))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(16408..16512)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agcttgtcat ctgccggatg aggcaaaacc ctgcctacgg cgcgattaca tcgtcccagc
       61 gcgatcgctc ttactgttga tggctcgtgc ttaaaaacaa tgcaaacttc accgtttcag
      121 ctggtgattt tcgactgtga tggtgtgctt gttgatagcg gaacgcatca ctaatcgcgt
      181 ctttgcagac atgctcaatg aactgggtct gttggtgact ttggatgaca tgtttgagca
      241 gtttgtgggt cattccatgg ctgactgtct caaactaatt gagcgacggt taggcaatcc
      301 tccaccccct gactttgttc agcactatca acgccgtacc cgtatcgcgt tagaaacgca
      361 tctacaagcc gttcctgggg ttgaagaggc tttggatgct cttgaattgc cctactgtgt
      421 tgcgtccagt ggtgatcatc aaaagatgcg aaccacactg agcctgacga agctctggcc
      481 acgatttgag ggacgaatct tcagcgtgac tgaagtacct cgcggcaagc catttcccga
      541 tgtctttttg ttggccgccg atcgcttcgg ggttaatcct acggcctgcg ctgtgatcga
      601 agacaccccc ttgggagtag cggcaggcgt ggcggcagga atgcaagtgt ttggctacgc
      661 gggttccatg cccgcttggc gtctgcaaga agccggtgcc catctcattt ttgacgatat
      721 gcgactgctg cccagtctgc tccaatcgtc gccaaaagat aactccacag cattgcccaa
      781 tccctaaccc ctgctcgcgc cgcaactaca cactaaaccg ttcctgcgcg atcgctctta
      841 ctgttgatgg ctcgtgctta aaaacaatgc aaccctaacc gtttcagctg gtgattttcg
      901 gacgatttgg cttacaggga taactgagag tcaacagcct ctgtccgtca ttgcacaccc
      961 atccatgcac tggggacttg actcatgctg aatcacattt cccttgtcca ttgggcgaga
     1021 ggggagggga atcttctgga ctcttcacta agcggcgatc gcaggttctt ctacccaagc
     1081 agtggcgatc gcttgattgc agtcttcaat gctggcctct gcagccatcg ccgccaccaa
     1141 agcatcgtag gcgggacgtt gttgctccag taaagtcttc gcccgtaaca atccccagcg
     1201 actgcgtaaa tccgcttcgg caggattgcg atcgagttgc cgccacagtt gtttccactg
     1261 ggcgcgatcg tcagctcccc cttccacgtt gccgtagacc agttgctctg ccgctgcacc
     1321 ggccatcaac acctgacacc actgttccag cgatcgctga ctgagttgcc cctgtgcggc
     1381 ttcggcttct agcgcagctg cttggaactg cacacccccg cgaccaggtt gtccttggcg
     1441 cagcgcttcc cacgctgaga gggtgtagcc cgtcacgggt aaccgatctg tttttgttcc
     1501 tgcaatgacc attgctgagg agttcccatg aaaatcaagt catcgatttc tgtgctcgct
     1561 gcgatgtttt cctgcttaac cgctgaaagt acgtccatat aaatgcctct attagttagc
     1621 gctatcggcg aaaagaatgg tgatataagg ggcatcgctg ccccttcagc atcagttaaa
     1681 cgttacgagt ggtatttgac gatgttggcg tttaccacat cgataaccgc tctttgctgc
     1741 gctttatctt ccatcgactc aaaagacatc aataggtccg cggcaccaca acactcaatc
     1801 gcaactcgcg ctgcctgagt actttcttca taagtaccga tggcattttc tgacaacagc
     1861 tctcccattt tttgctcaaa gtcggtattt gagtaagatt cacggacaaa ctcttccaaa
     1921 tacacgcgag cttctgccct tgctgcttca ccatctacat tttggttgac cagcagcgtc
     1981 agcttgtgtc gaacctgact aacatcgaca ccatgtgcct gagcaacttc gtggtacaaa
     2041 ccggcgtatt cttttctttg agcgtttgag tcgtcccatc taaacacgag tggaagccct
     2101 aacttagccg cccattcaac cacttctttg ctcgtcgcat tcacaaattg cgcaggaccg
     2161 ccttcagtga acgcgtgtgg gttaacggag attttaggaa aactataaaa atcattgttt
     2221 ggatggcagt acccagtagt gaatgcatca ttgatgatct tgtgacactc actgaacaac
     2281 tgaaactgag aatccgttgg tcgattaaag aagcgcatat ctgcactctt ttcacaatca
     2341 ctaaagccaa aggcaaaacg gccttcactc atttggtcaa gtagacacgc ttcttccgcc
     2401 acacgtactg gatgatgcgt ggtaatgacg tgattcaacg aagccacttt ggcgttcttt
     2461 gtcataccaa gtaaaaaacc agccactgtt agtggggcac caaccacacc attgttcgag
     2521 aaatggtttt cgtaaacagc caacgtgtca aacttcaact gatctacgta atgtgcggta
     2581 tctaacattt cttcgatgac ttgatcagaa gaacgctttg aattcataaa attgaggaag
     2641 aataatccaa atttcatgtc tctttccttt tagaaaatat taattactgt ttttctttga
     2701 gatatggcat cacatcagac tggaatagct tcatagatgc gataatttct tcttcagaac
     2761 cgtttgcttc aaaaccacaa caaatattgt caatacccgt cgcatcaata tcttgctgga
     2821 taatcgcgat acactcttca ggcgtcccta ctgggttgat ttcgtagctg taatcaattc
     2881 ggcgattggt gtctttgtgg cctttcaaca caaaatcacg ccattgacct ttattgaagt
     2941 cgtaaccttt tgtttggtca gagtcgtcaa aaatcttggt ggcattcacg tatgagtcgt
     3001 accaatggcc caagaagttg cggcaaatat ctttggctct atttgagtca tgatcgacgg
     3061 aggtgatgta agacaaacag tggtcaatct tagtcacatc gtagccatgt tcagtcgcga
     3121 cttcgttgta aagatcaagc tgcgctttct tctcgtgagt gttgatgatc cagcttagaa
     3181 tcattggtag gccacgctct gcagcccatt ctgtcgttga tgctgactcc gcgacgacat
     3241 aaacaggagc accaccttgt gtgtaagccg atggattcag ttggattttc gggaacttaa
     3301 tatgttcgtt atccgccgcg atatagcctt cattgaagcc ttctttcatc aagtcatacc
     3361 aacagtccat taaggctcgg ctgttatcca tgtctgtacc aaagacacga aaatctttat
     3421 cgtacaaacc gcgacaaata ccaaaacgga atcgtccttt tgacatttga tccagtaggt
     3481 ttacgtcttc tgcttgtcga accggatggg cagtcggcaa tacgatagct gcagtgccaa
     3541 cgttgagcgt ttctgtcgca cctaataggt gtgcggcagc aacataagga ttccctaaca
     3601 acccaaattc agtgaagtgg tgctctagca accaaacggt gtcgaagcca caaccttcag
     3661 acgctttgcc cagattaacc aatcgcttca tcacttcggt ctgagatagc tcaggtggct
     3721 gataagtgag aaggaagttt ccaaatttca taacatttcc ttatttgttg gtattacgat
     3781 aagacctaag ccatttctgg cgtacggctt tcaatttccg ctttcaagcg acgttcattc
     3841 acagtcgcga tggtgagttg ttcaaaatca ggctcgataa agtcgacggg aggggctgca
     3901 atgaacgcaa cgatgaagca gatggtggcg gtcagcagag tggggatcat cagcacgccg
     3961 aaccaaccca cgtagatgcg gttgtcggtg ctggttaccc actcacaaaa ccgatcccaa
     4021 acgttatcgc ggcgttgctc gcgaagaatg ctggtcatat cgatcttgag gttgtaaagg
     4081 gcaagagtct tagttaaaaa ctcttgcttt ttaggctagg agattaactg tcaaaaaggt
     4141 gtaaaaaacc cttaattttt agctaagtag acactatttt tatgtaacga aaactttgtg
     4201 aatttatgta atgtttagag cgatcgccca aggctacagt gctcaacgga gccaaaacta
     4261 ctgtctacac tggattagaa gacgctaaat ccagcctcta gacccctgct tggaactgca
     4321 cacccccgcg accaggttgt ccttggcgca gcgcttccca cgctgagagg gtgtagcccg
     4381 tcacgggtaa ccgatgggta ccgagctcga attgaattcc tttaatccct ttaattcctg
     4441 gattttttct ccgaaatctg ggagcgtata aacatctgga ctcgtataaa tcagttgact
     4501 ggtttctatg ccttctttgc cacatattat atgtatatca actaataccg tattattatc
     4561 ataacctata gacatagttc cattgtattt tagcgcgttc tcaaattctt cttttgatgg
     4621 atacgatgat ccatctccgt actttttcgt taacgatttg cttactggct tcacaagtct
     4681 gccaatatta atctctaatc ttttaaattt cccatttaaa aaaaatgcca ttgcttcacg
     4741 ctttttgtta gcaaacgaaa agtcagaaca tgaataaagt gatacttctt ctattcgaga
     4801 atctccctca aatttttcaa aatcacataa cctcttcgaa agaaactctt cttctgtcat
     4861 gccaaacttt gcatctttat acccatcaac tgccattgca ccaaagctaa tcattcccaa
     4921 tgcaagcata actgccaatt ttttcatgtt atctcctttt gctttgagcc aataataaca
     4981 atgagaacaa tgttaagcca accccataaa cctctatcaa gtacccaaat gtggatgttt
     5041 tggagaattt tgtaaagtgt agatgtcatt cagatttata taacgcaagc attccactta
     5101 caattaggca aaggatatca actatcaaac gcttcggtta agcttaaagc acaccctttc
     5161 tgcgtcctcg tattgacgcg acgtaaaatt tcaacgagca cgccgggata cttaccatat
     5221 tctctgctaa ttatcccgac atcatcggta acaataaatg ctggataact ggttgctgac
     5281 gcatccatat aactcatcaa ccccggcgtt ccatcaggta caggtttcaa cgtttcagga
     5341 tcaagcgctc gcgcatatac ccacggcgga acatgtttac gctgcatttc atcctcaaag
     5401 aaacaagtgt tgagttcaac ttgattaaat atatctcgga tctgactaat atcactgaga
     5461 ttgaaagtat caaataaaag atgattgaaa tcatcacgtt tcagagattc tttttcgtaa
     5521 cttttccagc cgcctccggt tatgatataa aggcttttat ctccagaaaa tgagattttt
     5581 ttatctttca tataatggca gagtaaataa ataaagtatg gcgaaccaat aagacaaaga
     5641 tctttccctt gattttttat tcgttcaaga ctattcaatg ttttaacaaa atctattcgt
     5701 tcttctgtta cggtaaatgt cgtaggatat aacaattcca ccaaactcat aacatattta
     5761 aaccaaatat tatgagcatt aaatctatct ggtcccaaat tgactaattc tatttgatga
     5821 tcaaaccaac taccaacata tttcatgcca taactcacag agcctaagag tctctcaata
     5881 cttaatctgt cacgcgccac ctgacttttt aaaccattcg tgccgctact ggtaaaccaa
     5941 ctttcaatct cgttttcctg agaagttaat aagcgagtaa acttaaaaac cgatgttggg
     6001 aatacaggta tgtcatcaat ttccgtaata ttgtcatcta ctttgtgtgc ctgacagtag
     6061 tgacgatatt ctcgacaatg tttataatga ttacgaaatg catcaagcac aagtttcttt
     6121 ctgatttttt cctgctcgtc gtaagaccac actaatggat cgctcgaaaa aatcaaatca
     6181 tcaatttctg agcttgctgt aatttcttgt ttatcaacat atgaagtcat acctgtttta
     6241 ctcctcaaga taatattaga aagtatggca gcactgctgt catactcttt tatacccttc
     6301 atctttcaag ctgctgcttt gttggctgct ttcactcacc ccagtcacat agttatctat
     6361 gctcctgggg attcgttcac ttgccgcctc gctgcaactc gaaatctatt aggtatattc
     6421 catgtggtac ttcttaatat tatcatcaac aatattgatt acattttttt ggctcatcaa
     6481 atcattcatt ggttcaaagg acagcaatac acttttcgca ccacactttt caattgccaa
     6541 cttagccgca gttatacact ccgtataatt tccgacagcg ttttctgcaa ttatttcttc
     6601 aagtttattt tcgaaatttt cattagggtg catttcaaga acataatcac taataaatgc
     6661 acgcggttac caagcaaacc aaactccgtg aaatgatgct ccagtaacca tacggtatca
     6721 aaaccacact cctcagagat gcgacctaat ttaaccaaac gtttcattac ctctgtttga
     6781 gaaaattggg gaggttggta tgtaagcaaa aagtttccaa atttcataga gagtccttat
     6841 attgctattt gagtgataga atatctcaat agattttaag acagagaaat tgcttgattt
     6901 tcaatctcaa ttctcattcg gcgttcattg actgtcgcaa tagttaaatg ttcaaatgac
     6961 ggttcagtaa tatcaacatc aatatccaga tgatcattat ccatcgcgat agcggctttc
     7021 gtaaccgatt gataaaaatt gcgcaggacc actaaatttt cactcaagtc atgcgaactt
     7081 cctaacaaag aatatatctt gcatcgatta ctacgaatat ttgataacaa tgtgataact
     7141 tcatcttgct tgacccaatt atcgttattt gcagtaaaag caataaacgg tatatcaaga
     7201 tacatcatgt tattaattgt agaagctaaa tcttcccaac caaaatcaag acaatctctc
     7261 gcaaagactt cagcacccaa tttatggcct tcaaaatcta gattattcgg caattcatta
     7321 atgggtagac tgagataatc aaaccctaaa gctctttcaa gagaatatct taagttaaca
     7381 acaccgactg cggtgattaa aaacgaagca ttgatttcag ataggcttgc ataagctatc
     7441 cgcgcagata agcttgaagc caacataccg aagttattta tttttcgtgt agttaaccaa
     7501 tcaaccactg ctaacaagct ctgctttcct atagacattg taaattcatc aattgtccct
     7561 gaactcaatc caacgtggtg aagcgaatca tagcggatca catgaaatcc attccgcgat
     7621 aaatattccg ccagaccagc aaaatgatcc atcctgcggg caaaaccaga cgcaataata
     7681 atggcattct ttctctttgg gctgttttct tctggcagcg tttcccaaac ataaattttt
     7741 ttatttcctt caacacaaat aacgtggtcg atggttttat attttgattc attttccata
     7801 ctttttacct attatgggac aaatacaagg aacttatctt cttccaggaa tcgagtctgt
     7861 tctatttcaa ccgcaacatc cttagccgta tagttagatg gcctttcatg agaaatatat
     7921 gtcactaatc gttgcaacgg tctcattccg tcatgagatc caccaactcg aaatatgtta
     7981 ttcattcctg cttctacaat cctttccgca ccttttaatg ctaacgcatc tcgatattta
     8041 aatgatgact cccaaggaaa aatagatatg gtttgcgtct tatttttttg aacataaggc
     8101 aatatttgct caatattatc gacgtgatga aggtacacac atctgccaag tggttgatta
     8161 aattccacac ctgcatttga ctcaataatc atccaacgtt gatgaatatc cacctctact
     8221 tttaatccag caaacaagct ttctttttga actaaagaat aggccgcctt ttcatcaaaa
     8281 tcttttttgg cattcggtaa tatatgcgca tatagattaa gtttttctat caacgctaac
     8341 ttaaattcct cataatgatt tcccatgtaa tatatgtttt gggcagaaaa acaagctcgc
     8401 tgatcgtaaa aacaaacatc atgagccgca cctgtcgctg cggacgtcaa atcaacagga
     8461 ttatcgataa tgcaaagact ctttttagaa ccaaatttaa tcacatcagc ataagatggc
     8521 gcatgctcta ccgcccaatt aatcgcatct ggccctcccc aagcgacaat aacatccgca
     8581 tgtcgcataa tttcttttgc gagtgatgta tcaccttggt ggggccaata tataacagat
     8641 aaagagcgcg ttatcggatg attagggtct acatcaataa aacttaacgc taatgcatta
     8701 gcggtaaaag gatcggttga cgatgttttt ataatacact gattcttagt taaaattgcg
     8761 cgtaatatag acatgatccc agataatgga acattacctg ccaacagatg tacagattta
     8821 cctttcggaa aagcccgaac ataactttca tcctgaggta gccattcatc catgatatgg
     8881 cgagaaccaa gttcattttc tacaacatca taaaggccgc ctttagaaca taaaatcata
     8941 gatatccaat tggcctctag cttagccatt tcttctgaat atcccatata tttttttaag
     9001 tcacgaatgt atgtcctgcg tcttgagtat tcttcatttt tccatctttg ccctaccgta
     9061 tagagaaaat tgacaatgtt atgcaaccgt aattcgttat ttccattaca atcaataatg
     9121 ttttttacat gagagtcatt caatattggc aggtaaacac tattatcacc aaaattaatg
     9181 gattgcacta aatcatcact ttcgggaaag atttcaacct ggccgttaat aatgaatgaa
     9241 atttttttag tcatatttgc catccacgag ctcggtaccc atcgtcgacg ggaggggctg
     9301 caatgaacgc aacgatgaag cagatggtgg cggtcagcag agtggggatc atcagcacgc
     9361 cgaaccaacc cacgtagatg cggttgtcgg tgctggttac ccactcacaa aaccgatccc
     9421 aaacgttatc gcggcgttgc tcgcgaagaa tgctggtcat atcgatcttg aggttgtaaa
     9481 gggcaagagt cttagttaaa aactcttgct ttttaggcta ggagattaac tgtcaaaaag
     9541 gtgtaaaaaa cccttaattt ttagctaagt agacactatt tttatgtaac gaaaactttg
     9601 tgaatttatg taatgtttag agcgatcgcc caaggctaca gtgctcaacg gagccaaaac
     9661 tactgtctac actggattag aagacgctaa atccagcctc tagacccggg ctcgagctag
     9721 caagcttggc cggatccggc cggatccggg agtttgtaga aacgcaaaaa ggccatccgt
     9781 caggatggcc ttctgcttaa tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     9841 ccctccgggc cgttgcttcg caacgttcaa atccgctccc ggcggatttg tcctactcag
     9901 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
     9961 tcgttttatt tgatgcctgg cagttcccta ctctcgcatg gggagacccc acactaccat
    10021 cggcgctacg gcgtttcact tctgagttcg gcatggggtc aggtgggacc accgcgctac
    10081 tgccgccagg caaattctgt tttattgagc cgttacccca cctactagct aatcccatct
    10141 gggcacatcc gatggcaaga ggcccgaagg tccccctctt tggtcttgcg acgttatgcg
    10201 gtattagcta ccgtttccag tagttatccc cctccatcag gcagtttccc agacattact
    10261 cacccgtccg ccactcgtca gcaaagaagc aagcttagat cgatccgtca cacgggataa
    10321 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10381 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10441 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10501 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10621 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10681 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    10741 gaggcccttt cgtcttcgaa taaatacctg tgacggaaga tcacttcgca gaataaataa
    10801 atcctggtgt ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg
    10861 ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
    10921 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
    10981 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
    11041 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
    11101 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
    11161 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
    11221 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
    11281 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
    11341 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
    11401 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
    11461 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
    11521 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
    11581 atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt
    11641 attggtgccc ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg
    11701 gcagaaattc gaaagcaaat tcgacccggt cgtcggttca gggcagggtc gttaaatagc
    11761 cgcttatgtc tattgctggt ttaccggttt attgactacc ggaagcagtg tgaccgtgtg
    11821 cttctcaaat gcctgaggcc agtttgctca ggctctcccc gtggaggtaa taattgacga
    11881 tatgatcgac ggatctggta accccagcgc ggttgctacc aagtagtgac ccgcttcgtg
    11941 atgcaaaatc cgctgacgat attcgggcga tcgctgctga atgccatcga gcagtaacgt
    12001 ggcaccccgc ccctgccaag tcaccgcatc cagactgaac agcaccaaga ggctaaaacc
    12061 caatcccgcc ggtagcagcg gagaactacc cagcattggt cccaccaaag ctaatgccgt
    12121 cgtggtaaaa atcgcgatcg ccgtcagact caagcccagt tcgctcatgc ttcctcatct
    12181 aggtcacagt cttcggcgat cgcatcgatc tgatgctgca gcaagcgttt tccataccgg
    12241 cgatcgcgcc gtcgcccttt cgctgccgtg gcccgcttac gagctcgttt atcgaccacg
    12301 atcgcatcca aatccgcgat cgcttcccag tccggcaatt cagtctgggg cgtccgtttc
    12361 attaatcctg atcaggcacg aaattgctgt gcgtagtatc gcgcatagcg gccagcctct
    12421 gccaacagcg catcgtgatt gcctgcctca acaatctggc cgcgctccat caccaagatg
    12481 cggctggcat tacgaaccgt agccagacgg tgagcaatga taaagaccgt ccgtccctgc
    12541 atcacccgtt ctagggcctc ttgcaccaag gtttcggact cggaatcaag cgccgaagtc
    12601 gcctcatcca gaattaaaat gcgtggatcc tctacgccgg acgcatcgtg gccggcatca
    12661 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
    12721 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
    12781 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
    12841 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
    12901 gtcgatcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
    12961 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
    13021 gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg
    13081 atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact
    13141 ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac
    13201 gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg
    13261 attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag
    13321 gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact
    13381 tcgatcactg gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac
    13441 gggttggcat ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc
    13501 ggtgcatgga gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat
    13561 tcaccactcc aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca
    13621 acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct
    13681 cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc
    13741 ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa
    13801 cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg
    13861 gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc
    13921 ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga
    13981 agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt
    14041 gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga
    14101 gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg
    14161 aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc
    14221 agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct
    14281 gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg
    14341 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
    14401 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
    14461 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
    14521 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    14581 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    14641 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    14701 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    14761 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    14821 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    14881 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    14941 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    15001 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    15061 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    15121 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    15181 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    15241 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    15301 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    15361 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    15421 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    15481 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    15541 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    15601 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    15661 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    15721 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    15781 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    15841 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    15901 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    15961 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    16021 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    16081 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    16141 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
    16201 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    16261 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    16321 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    16381 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    16441 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    16501 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
    16561 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaatt
//
