LOCUS       sequence-51672-e        5819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Ditty JL, Canales SR, Anderson BE, Williams SB, Golden SS
  TITLE     Stability of the Synechococcus elongatus PCC 7942 circadian clock 
            under directed anti-phase expression of the kai genes.
  JOURNAL   Microbiology. 2005 Aug;151(Pt 8):2605-13.
  PUBMED    16079339
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology."; date: "2005-08"; volume: "151(Pt 8)"; pages: 
            "2605-13"
COMMENT     Sequence Label: pAM2314
FEATURES             Location/Qualifiers
     source          1..5819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      725..1313
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1214..1233
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1467..1484
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1499..1639
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1565..1584)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1725..1747
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             3558..4349
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
       61 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
      121 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
      181 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
      241 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
      301 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
      361 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
      421 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
      481 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
      541 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
      601 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
      661 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
      721 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      781 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      841 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
      901 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      961 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1021 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1081 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1141 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1201 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1261 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1321 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1381 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     1441 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     1501 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     1561 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     1621 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     1681 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     1741 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag gatcgatccg gcagccggcg
     1801 gagcgctgct ttcttggcaa gcggtcgcca gccccaacgc cagggctgcc agcccgaaac
     1861 agcggggcaa ggcagcttgg aagggcgatc gcagcacggg catggcaatg tctctctgaa
     1921 ggaatgcaga ccttattcgt acagccaggg ttgaatcgtg ggggtccaat cacttagctc
     1981 tgctgggcta aaccagagag caatttcctg ttgtgctgtt tcgattgcat ccgagccatg
     2041 gatgatgttg cggccaatat tgacaccaaa atcaccacgg atggtgcccg gttctgccgt
     2101 cagcggattg gtagcgccga tcaacttgcg agcagccgcc acaacgcctt cgccttccaa
     2161 gacgatcgcc acgatcggcc cagaggtgat gaactcgacg aggccattga agaaggggcg
     2221 ctcgcggtgg acagcatagt gctgttcggc cagctcgcga ctgggcttca gctgctttag
     2281 gcccaccagt ttgaagcctt tttgctcaaa gcggccgatg atcgtaccga ccaaaccccg
     2341 ctgaacgcca tcgggcttga tggcaataaa tgtgcgttcc acagacatct agatagtcct
     2401 caagacgagg caagcattga gcttgccttc ctatggttcg ggatcactgg gattcttgac
     2461 aagcgatcgc ggtcacatcg ctatctctta ggacttcgca gcgggcgagt cggattgacc
     2521 cggtagggat ttcgccagat caatgcccgt ggtttgtttc agcttctcca gcaagctagc
     2581 gatttgggta gcgctgcctt ccccttcgcc aatcacagtg atcgactcca cgtcgatatc
     2641 tggcacggtg cctgaaagcg tgacgagcag ggactcgaag cttgcatgcc tgcaggtcga
     2701 ctctagagct ttatgcttgt aaaccgtttt gtgaaaaaat ttttaaaata aaaaagggga
     2761 cctctagggt ccccaattaa ttagtaatat aatctattaa aggtcattca aaaggtcatc
     2821 caccggatca attcccctgc tcgcgcaggc tgggtgccaa gctctcgggt aacatcaagg
     2881 cccgatcctt ggagcccttg ccctcccgca cgatgatcgt gccgtgatcg aaatccagat
     2941 ccttgacccg cagttgcaaa ccctcactga tccgcatgcc cgttccatac agaagctggg
     3001 cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg aaccacttca tccggggtca
     3061 gcaccaccgg caagcgccgc gacggccgag gtcttccgat ctcctgaagc cagggcagat
     3121 ccgtgcacag caccttgccg tagaagaaca gcaaggccgc caatgcctga cgatgcgtgg
     3181 agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc
     3241 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     3301 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     3361 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     3421 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     3481 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     3541 aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag
     3601 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     3661 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     3721 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     3781 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     3841 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     3901 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     3961 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     4021 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     4081 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     4141 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     4201 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     4261 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     4321 gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct
     4381 tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa
     4441 ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt
     4501 gggagatata tcatgaaagg ctggcttttt cttgttatcg caatagttgg cgaagtaatc
     4561 gcaacatccg cattaaaatc tagcgagggc tttactaagc tgatccggtg gatgaccttt
     4621 tgaatgacct ttaatagatt atattactaa ttaattgggg accctagagg tccccttttt
     4681 tattttaaaa attttttcac aaaacggttt acaagcatac tagaggatcg gcggccgcgg
     4741 agatctacgt acgcgtgaat tcctcgaggc ctactagtcg gatccccggg taccgagctc
     4801 gaatttcgag cttctggagc aggaagatgt cgcgggcatt agcaccagcg gtctgccaag
     4861 cctccgccag ccgttgggtc ccttccgctt gagcttttcc atcttcgacg atacgggcgg
     4921 cggccccccg cgcttccgcg atcgcccgtt tacaagctgc ctcagctggg gcgatcacat
     4981 cggcttgaag ttgctgctgc acctgtttga tccgctcctg ctgcacaggg agttctgctt
     5041 ggctacgagc gacttcggta gcaatgtccg cttcagcttc ggccaccacc gcttcgcgcc
     5101 gcgtcaacgc atcctgaatc cggcgctcgg cctcggcttg ggcgatcgct acatcgcgat
     5161 cgatccgacg cagggccgtg atcttgtcat tttcggccgt ttggatcgca gaggcagcct
     5221 gggcatcggc ttcagcaatt cgggcatctc gctgcagatc agcccgctgc ttgcgtccac
     5281 tagccgagag ataaccgacc tcatcggaaa tgttctggac ttgcagcgta tcgaggacta
     5341 gacccagctg ctcaaggtca tcctccgcct cttccagcag acttttggca aaggcaattt
     5401 tgtcctcgtt gatctgctcc ggcgtgaggc tggctaaaac accacgcaag ttgccttcga
     5461 gggtctcctt ggcaatttgc tcgatttcct tacggttttt gccaagcagc cgctcgatcg
     5521 cgttgtggat ggtcggttct tccccagcaa tcttgatatt ggcaacgcct tcaacagtca
     5581 ggggaatgcc gcccttggag aaggcattgg aaacgcgcaa ctcaatgatc atgttggtca
     5641 gatccatgcg gagcgctttt tccagcagag gtacccgcag gctgctgccg cccttgacca
     5701 agcgatagcc aactcggcgg ccatcactac tgcggcgact actgccagca aagatcaaaa
     5761 tttcactggg ttggcagatg tagtagagat tgcgcaggac taagctgcca gccccggcg
//
