LOCUS       sequence-51681-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DLG3
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..46
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..91
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             460..477
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(530..548)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      544..591
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(715..734)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttaactttaa gaaggagata tacatatgca ccatcatcat catcattctt ctggtgtaga
       61 tctgggtacc gagaacctgt acttccaatc catgttcaaa tatgaggaaa tcgtacttga
      121 gaggggcaac tctggcctgg gcttcagtat cgcaggtggc atcgacaatc cccatgtccc
      181 tgatgaccct ggcatcttta ttaccaagat tatccctggt ggagcagctg ccatggatgg
      241 gaggctgggg gtgaatgact gtgtgctgcg ggtgaatgag gtggacgtgt cggaggtggt
      301 acacagccgg gcggtggagg cgctgaagga ggcaggccct gtggtgcgat tggtggtgcg
      361 gaggcgacag cctccacccg aggaaacttc tgtttgacag taaaggtgga tacggatccg
      421 aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca ccaccactga
      481 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      541 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
      601 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
      661 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      721 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      781 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
      841 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
      901 cgtt
//
