LOCUS       sequence-51682-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SLC9A3R2
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(349..369)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(394..411)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             422..444
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    459..483
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(483..502)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(484..502)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(586..605)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggtggtggtg gtggtgctcg agtgcggccg caagcttgtc gacggagctc gaattcggat
       61 ccgtatccac ctttactgtc aaagacgagt actggggccc acgaccagca gccgggcctc
      121 gtcctcccgt gccttgatgc tggccaccac ctcagcatgg cgcagtccct ccacattctg
      181 cccgttcacc tcaatgagcc ggtcctgggc gcggaggcca gagcgggcgg caggtgagcc
      241 cgggtccaca gagcggatgt actggccggg ccgggacttg tcactatgca ggttgaaccc
      301 atagccctga ggtccctttc gcaggtggca gagccgaggg cgcagcatgg attggaagta
      361 caggttctcg gtacccagat ctacaccaga agaatgatga tgatgatggt gcatatgtat
      421 atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc cgctcacaat
      481 tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac
      541 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
      601 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
      661 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
      721 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgt
//
