LOCUS       sequence-51683-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SLC9A3R2
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             58..78
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             411..428
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(481..499)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      495..542
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(666..685)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct gggtaccgag
       61 aacctgtact tccaatccat gctgcgccct cggctctgcc acctgcgaaa gggacctcag
      121 ggctatgggt tcaacctgca tagtgacaag tcccggcccg gccagtacat ccgctctgtg
      181 gacccgggct cacctgccgc ccgctctggc ctccgcgccc aggaccggct cattgaggtg
      241 aacgggcaga atgtggaggg actgcgccat gctgaggtgg tggccagcat caaggcacgg
      301 gaggacgagg cccggctgct ggtcgtgggc cccagtactc gtctttgaca gtaaaggtgg
      361 atacggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc
      421 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      481 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      541 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
      601 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      661 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
      721 agctctaaat
//
