LOCUS       sequence-51687-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SLC9A3R1
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..36
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..81
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             428..445
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(498..516)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      512..559
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(683..702)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gaaggagata tacatatgca ccatcatcat catcattctt ctggtgtaga tctgggtacc
       61 gagaacctgt acttccaatc catgcttcgg cctcggctct gtaccatgaa gaagggcccc
      121 agtggctatg gcttcaacct gcacagcgac aagtccaagc caggccagtt catccggtca
      181 gtggacccag actccccggc tgaggcttca gggctccggg cccaggatcg cattgtggag
      241 gtgaacgggg tctgcatgga ggggaagcag catggggacg tggtgtccgc catcagggct
      301 ggcggggacg agaccaagct gctggtggtg gacagggaaa ctgacgagtt cgaaacttct
      361 ctttgacgta aaggtggata cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc
      421 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
      481 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      541 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg
      601 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      661 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      721 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
      781 ttacggc
//
