LOCUS       sequence-51689-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DLG3
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             96..116
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             461..478
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(531..549)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      545..592
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(716..735)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgggtaattc cctctgaata ttttgtttac tttaagaagg agatatacat atgcaccatc
       61 atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc caatccatga
      121 ccatcatgga ggtcaacctg ctcaaagggc ccaaaggcct gggtttcagc attgctgggg
      181 gtattggcaa ccagcacatc ccaggagaca acagcatcta catcaccaag atcattgagg
      241 ggggtgctgc tcagaaggat ggacgcctac agattgggga ccggctgctg gcggtgaaca
      301 acaccaatct gcaggatgtg aggcacgagg aagctgtggc ctcactgaag aacacatctg
      361 atatggtgta tttgaaggtg gccaagccag gcagctgaca gtaaaggtgg atacggatcc
      421 gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg
      481 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      541 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
      601 aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
      661 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
      721 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
      781 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc aaaaaacttg
      841 attagggtga tggttcacgt agtgggccat cgcccttgat agacggtttt ttcgcccttt
      901 gacgttggag tccacgttct tttaatagtg gaac
//
