LOCUS       sequence-51690-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS3
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(378..398)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(423..440)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             451..473
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    488..512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(512..531)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(513..531)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(615..634)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag
       61 cttgtcgacg gagctcgaat tcggatccgt atccaccttt actgtcaatc tggtcctggc
      121 ttgacctggg ggaccatgcg ccacacgagt aggatgatct cactggggca gctccggatc
      181 tcgtgggcca gctccacaca tttccagtgc tccacaggcc tctcattcag ctgcagcacc
      241 gtgtccagct gctgcagccc tgcccgttcc gccggacccc cggaatccac ggcctggact
      301 cgaactggag agtcgcagca gatggtgaag ccaaagccgt cctttcccct cgggatggtg
      361 atctggcggt agcgcatgga ttggaagtac aggttctcgg tacccagatc tacaccagaa
      421 gaatgatgat gatgatggtg catatgtata tctccttctt aaagttaaac aaaattattt
      481 ctagagggga attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg
      541 gatcgagatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
      601 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
      661 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
      721 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
      781 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gagatcccgg
      841 acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgccc
//
