LOCUS       sequence-51692-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: YWHAQ
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             867..884
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tattttgttt aactttaaga aggagatata catatgcacc atcatcatca tcattcttct
       61 ggtgtagatc tgggtaccga gaacctgtac ttccaatcca tggagaagac tgagctgatc
      121 cagaaggcca agctggccga gcaggccgag cgctacgacg acatggccac ctgcatgaag
      181 gcagtgaccg agcagggcgc cgagctgtcc aacgaggagc gcaacctgct ctccgtggcc
      241 tacaagaacg tggtcggggg ccgcaggtcc gcctggaggg tcatctctag catcgagcag
      301 aagaccgaca cctccgacaa gaagttgcag ctgattaagg actatcggga gaaagtggag
      361 tccgagctga gatccatctg caccacggtg ctggaattgt tggataaata tttaatagcc
      421 aatgcaacta atccagagag taaggtcttc tatctgaaaa tgaagggtga ttacttccgg
      481 taccttgctg aagttgcgtg tggtgatgat cgaaaacaaa cgatagataa ttcccaagga
      541 gcttaccaag aggcatttga tataagcaag aaagagatgc aacccacaca cccaatccgc
      601 ctggggcttg ctcttaactt ttctgtattt tactatgaga ttcttaataa cccagagctt
      661 gcctgcacgc tggctaaaac ggcttttgat gaggccattg ctgaacttga tacactgaat
      721 gaagactcat acaaagacag caccctcatc atgcagttgc ttagagacaa cctaacactt
      781 tggacatcag acagtgcagg atgacagtaa aggtggatac ggatccgaat tcgagctccg
      841 tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
      901 acaaagcccg aaaggaagct ga
//
