LOCUS       sequence-51693-e         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS6
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(365..385)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(410..427)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             438..460
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    475..499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(499..518)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(500..518)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(602..621)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgaccggag gaagcgggca tagctgtcac tcttcatcag cttgtagatg tgctcctggg
       61 cgtcttcaaa tgtatatctc cctccatctt tgacattttg actggttatc tcatagctgt
      121 gagaatccag gttgattgca cttggagccc ctggagccag aaactcttgc cagatttctt
      181 ctaccctctt ggccacatcc tgtaggggtt gtttcttaag atcttggaca gccagccaga
      241 acctgaggtt ttctgaactg aattcggact ccaggaatcg tagaaactgg tcccgcccca
      301 cctggtcctt caatatctca tcgaaagaga agccccatct ttttactcgc tgttggctgg
      361 gcatggattg gaagtacagg ttctcggtac ccagatctac accagaagaa tgatgatgat
      421 gatggtgcat atgtatatct ccttcttaaa gttaaacaaa attatttcta gaggggaatt
      481 gttatccgct cacaattccc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg
      541 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
      601 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
      661 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg gggg
//
