LOCUS       sequence-51694-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS6
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             605..622
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(675..693)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      689..736
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gcccagccaa cagcgagtaa
      121 aaagatgggg cttctctttc gatgagatat tgaaggacca ggtggggcgg gaccagtttc
      181 tacgattcct ggagtccgaa ttcagttcag aaaacctcag gttctggctg gctgtccaag
      241 atcttaagaa acaaccccta caggatgtgg ccaagagggt agaagaaatc tggcaagagt
      301 ttctggctcc aggggctcca agtgcaatca acctggattc tcacagctat gagataacca
      361 gtcaaaatgt caaagatgga gggagatata catttgaaga cgcccaggag cacatctaca
      421 agctgatgaa gagtgacagc tatgcccgct tcctccggtc aaatgcttac caggatttgc
      481 tgctggccaa gaagaaggga aagtcgctgg cgggcaagcg cctcacgggc ctgatgcagt
      541 gacagtaaag gtggatacgg atccgaattc gagctccgtc gacaagcttg cggccgcact
      601 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
      661 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
      721 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
      781 tgtagcggc
//
