LOCUS       sequence-51705-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MAP3K3
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(381..401)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(426..443)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             454..476
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    491..515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(515..534)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(516..534)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(618..637)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atccgtatcc acctttactg tcagtcctgg
      121 gacaacagca atatcctaag gcttttcatg cttgagcttc tatctaaaat gtcaattgct
      181 ttatcaagat catcttggtt tttcagcagg atggagagct cattgttcat gtaatgtaga
      241 tcaagaggtt gtccaaatac tgttgtcacc ttgtgctcca catcttcata tttcacaggc
      301 cggctgaacg ctataattcg cctctccccg ttgtgctcga acttgattct gacgtcactc
      361 tgcctatttg agtgacccat ggattggaag tacaggttct cggtacccag atctacacca
      421 gaagaatgat gatgatgatg gtgcatatgt atatctcctt cttaaagtta aacaaaatta
      481 tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt attaatttcg
      541 cgggatcgag atctcgatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
      601 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
      661 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
      721 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
      781 caacctacta ctggg
//
