LOCUS       sequence-51707-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MAP3K3
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             431..448
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(501..519)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      515..562
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(686..705)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gggtcactca aataggcaga
      121 gtgacgtcag aatcaagttc gagcacaacg gggagaggcg aattatagcg ttcagccggc
      181 ctgtgaaata tgaagatgtg gagcacaagg tgacaacagt atttggacaa cctcttgatc
      241 tacattacat gaacaatgag ctctccatcc tgctgaaaaa ccaagatgat cttgataaag
      301 caattgacat tttagataga agctcaagca tgaaaagcct taggatattg ctgttgtccc
      361 aggactgaca gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca agcttgcggc
      421 cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga
      481 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
      541 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac
      601 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
      661 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      721 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      781 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
      841 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttc
//
