LOCUS       sequence-51709-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PRKCG
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(540..560)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(585..602)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             613..635
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    650..674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(674..693)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(675..693)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(777..796)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattcggat ccgtatccac ctttacttca gttgtcagca
      121 tcggccaccg gcacattgta atactcgccc tcctcctggt tcagtaactt gtaccagcca
      181 tccacgggcg ccttgagcag ctccgagacg ccaaaggaca tggcccccat gaagtcgttg
      241 cgggaggtcc ggtcccagtc ccacacctcc acgctgagcc ggcgctccac atcccctggc
      301 ttcaggttga acacaaaggt ctcattccac acagggttta gcgtggcttt caccgttcgg
      361 gtcttctgtt tcgtcaggtt ccgagggtct gggatgagct tcagtttcac atagggatca
      421 gagagaccgt tggggtccat aggaattagg ttacgggcct cgccaacagt tacgtggatc
      481 tcatctgctg tgggagcccg gatctccagc tgcaggcgcc cgcggcgctc ggtgtgcatg
      541 gattggaagt acaggttctc ggtacccaga tctacaccag aagaatgatg atgatgatgg
      601 tgcatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat
      661 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct
      721 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
      781 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcggg
//
