LOCUS       sequence-51711-e         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PRKCG
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..24
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             518..535
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(588..606)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      602..649
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 accgagaacc tgtacttcca atccatgcac accgagcgcc gcgggcgcct gcagctggag
       61 atccgggctc ccacagcaga tgagatccac gtaactgttg gcgaggcccg taacctaatt
      121 cctatggacc ccaacggtct ctctgatccc tatgtgaaac tgaagctcat cccagaccct
      181 cggaacctga cgaaacagaa gacccgaacg gtgaaagcca cgctaaaccc tgtgtggaat
      241 gagacctttg tgttcaacct gaagccaggg gatgtggagc gccggctcag cgtggaggtg
      301 tgggactggg accggacctc ccgcaacgac ttcatggggg ccatgtcctt tggcgtctcg
      361 gagctgctca aggcgcccgt ggatggctgg tacaagttac tgaaccagga ggagggcgag
      421 tattacaatg tgccggtggc cgatgctgac aactgaagta aaggtggata cggatccgaa
      481 ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac caccaccacc accactgaga
      541 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      601 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaa
//
