LOCUS       sequence-51717-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CSNK1G3
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gggagtttta atggttggac
      121 ctaactttag agttggaaaa aaaattggat gtggcaattt tggagaatta cgattaggga
      181 aaaatttata cacaaatgaa tatgtggcaa ttaagttgga gcccatgaaa tcaagagcac
      241 cacagctaca tttggaatac agattctata agcagttagg atctggagat ggtatacctc
      301 aagtttacta tttcggccct tgtggtaaat acaatgctat ggtgctggaa ctgctgggac
      361 ctagtttgga agacttgttt gacttgtgtg acagaacatt ttctcttaaa acagttctca
      421 tgatagctat acaactgatt tctcgcatgg aatatgtcca ttcaaagaac ttgatataca
      481 gagatgtaaa acctgagaac ttcttaatag gacgaccagg aaacaaaacc cagcaagtta
      541 ttcacattat agattttgct ttggcaaagg aatatattga tccggagaca aagaaacaca
      601 taccatacag agaacacaag agccttacag gaacagctag atatatgagc ataaacacac
      661 atttaggaaa agaacaaagt agaagagacg atttagaagc tttaggtcat atgttcatgt
      721 attttctgag aggcagtctt ccttggcaag gcttaaaggc tgacacatta aaggagaggt
      781 atcagaaaat tggagataca aaacgggcta caccaataga agtgttatgt gaaaattttc
      841 cagaaatggc aacatatctt cgttatgtaa gaaggctaga tttttttgaa aa
//
