LOCUS       sequence-51718-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BRD3
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             542..559
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(612..630)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      626..673
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(798..817)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgccggagg
      121 tctccaaccc cagcaagccc ggccgcaaga ccaaccagct gcagtacatg cagaatgtgg
      181 tggtgaagac gctctggaaa caccagttcg cctggccctt ctaccagccc gtggacgcaa
      241 tcaaattgaa cctgccggat tatcataaaa taattaaaaa cccaatggat atggggacta
      301 ttaagaagag actagaaaat aattattatt ggagtgcaag cgaatgtatg caggacttca
      361 acaccatgtt tacaaattgt tacatttata acaagcccac agatgacata gtgctaatgg
      421 cccaagcttt agagaaaatt tttctacaaa aagtggccca gatgccccaa gaggaatgac
      481 agtaaaggtg gatacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga
      541 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
      601 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      661 gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt
      721 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      781 agcgccccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
      841 ctttccccgt caagctctaa atcgggctcc ttaggtcgat
//
