LOCUS       sequence-51720-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BRD3
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             21..43
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             96..116
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             518..535
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(588..606)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      602..649
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(773..792)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacat atgcaccatc
       61 atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc caatccatgg
      121 gcaagctgtc ggagcaccta cgctactgcg acagcatcct cagggagatg ctatccaaga
      181 agcacgcggc ctacgcctgg cccttctaca agccagtgga tgccgaggcc ctggagctgc
      241 acgactacca cgacatcatc aagcacccga tggacctcag caccgtgaaa aggaagatgg
      301 atggccgaga gtacccagac gcacagggct ttgctgctga tgtccggctg atgttctcga
      361 attgctacaa atacaatccc ccagaccacg aggttgtggc catggcccgg aagctccagg
      421 acgtgtttga gatgaggttt gccaagatgc catgacagta aaggtggata cggatccgaa
      481 ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac caccaccacc accactgaga
      541 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      601 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      661 aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
      721 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      781 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
      841 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aacttgatta
      901 gggtgatggt tcacgtagtg ggccatcgcc ctgataga
//
