LOCUS       sequence-51723-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BRD4
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             546..563
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(616..634)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      630..677
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgaaccc cccgccccca
      121 gagacctcca accctaacaa gcccaagagg cagaccaacc aactgcaata cctgctcaga
      181 gtggtgctca agacactatg gaaacaccag tttgcatggc ctttccagca gcctgtggat
      241 gccgtcaagc tgaacctccc tgattactat aagatcatta aaacgcctat ggatatggga
      301 acaataaaga agcgcttgga aaacaactat tactggaatg ctcaggaatg tatccaggac
      361 ttcaacacta tgtttacaaa ttgttacatc tacaacaagc ctggagatga catagtctta
      421 atggcagaag ctctggaaaa gctcttcttg caaaaaataa atgagctacc cacagaagaa
      481 tgacagtaaa ggtggatacg gatccgaatt cgagctccgt cgacaagctt gcggccgcac
      541 tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg
      601 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
      661 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc
      721 ctgtagcggc gcattaagcg cggc
//
