LOCUS       sequence-51724-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BRD4
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(491..511)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(536..553)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             564..586
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    601..625
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(625..644)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(626..644)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(728..747)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcgg atccgtatcc acctttactg tcactcgtcc ggcatcttgg
      121 caaagcgcat ttcgaacaca tcctggagct tgcgggccat ggccaccacc tcatggtcag
      181 gagggttgta cttatagcag ttggagaaca tcaatcggac gtcagcacca aactcctgag
      241 catcacggta ctcacgggcc tccagtttag acttgattgt gctcatgtcc atggggtgct
      301 tgatgatgtc acagtagtcg tgtaggccca gtgcctccac gtccacaggc ttgtagaagg
      361 gccaggcgta ggcggcgtgc ttcttggcaa acatctcctt gaggatgccg ctgcagcact
      421 tgagctgctc cgagaccttg ctgctcttct ctggtgctgg gtgctgctga gagtcgggca
      481 cgtccttcat ggattggaag tacaggttct cggtacccag atctacacca gaagaatgat
      541 gatgatgatg gtgcatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg
      601 ggaattgtta tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag
      661 atctcgatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
      721 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
      781 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
      841 gccatct
//
