LOCUS       sequence-51725-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BRD4
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             550..567
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(620..638)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      634..681
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(805..824)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg
       61 tgtagatctg ggtaccgaga acctgtactt ccaatccatg aaggacgtgc ccgactctca
      121 gcagcaccca gcaccagaga agagcagcaa ggtctcggag cagctcaagt gctgcagcgg
      181 catcctcaag gagatgtttg ccaagaagca cgccgcctac gcctggccct tctacaagcc
      241 tgtggacgtg gaggcactgg gcctacacga ctactgtgac atcatcaagc accccatgga
      301 catgagcaca atcaagtcta aactggaggc ccgtgagtac cgtgatgctc aggagtttgg
      361 tgctgacgtc cgattgatgt tctccaactg ctataagtac aaccctcctg accatgaggt
      421 ggtggccatg gcccgcaagc tccaggatgt gttcgaaatg cgctttgcca agatgccgga
      481 cgagtgacag taaaggtgga tacggatccg aattcgagct ccgtcgacaa gcttgcggcc
      541 gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa
      601 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      661 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg
      721 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      781 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
      841 tcgccggctt tccccgtcaa gctctaaatc gggggctccc
//
