LOCUS       sequence-51728-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00522
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(748..765)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
       61 ctttcttata atgccaactt tgtacaagaa agttggcaag aggtagaatt ccggagagct
      121 ttggctatcg ccgccggcgg ttgcacctcc cgcaccgact ccgccatttg ccgcccgctg
      181 cagacgctgc atgagggcat cccgctcctg gcagaccctc gagtactcca gcttcaggcg
      241 gtgcagatcc tggttgagca cccggttggc cttctccagt tcgtggcgct gatggagtcg
      301 cttggaccgg cagttctggg catagccgcg attcttcagg gtgcgtcgct tctgcttcag
      361 acgcactacc tcctcccgtg ggcaaccatg gagccgcttg ttcagctccc gcaccgtcaa
      421 cgtggtcagc atgtcgtcat tgaggcaatc ctccagcgtg gcattactgt aggcacccga
      481 acaggttctg ggcgacatgt tgttcgagga tctggtcgag ctggcggaga gcgggcggca
      541 cggctgaagt gcagcccgtg tgatgacgca ggagccgcca cccacgctgc cgggtccacc
      601 gctcccgctt ccactgcctc ctccgcctcc gctgctgcca ctcccgttgc tgggcacttg
      661 gggcagacag gtgggcgtca gggcacccga agcactaccg tacgagttcg agtgcatgtt
      721 gatgggcgtc agttccagat tgtggaagtg gtggtgatgg tggtgcggat acttgctctg
      781 ctgcagcatc acctggtggg gatgaccgcc gtgtccatgc g
//
