LOCUS       sequence-51729-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MPDZ
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(391..411)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(436..453)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             464..486
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    501..525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(525..544)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(526..544)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(628..647)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc gtatccacct ttactgtcaa agacgagtac ttttgtatgg
      121 ggcctcatct ctgtagagtg tcaggcgcac tctctgtggc gtctgtctca ggacattgat
      181 tgcttcatca tgtgtggcct ttctcaagtc aattccattc acctctaaga tctgatctcc
      241 agcccagagt cttccatctt tacatgctgc tccttcttca taaacttcat ggataataat
      301 ggcacccagc agcgtgtctg aacccccaac gatgctcagg cccagccctg ttcgcccttt
      361 ggaaatctcg atggttgttt cgcagcccat ggattggaag tacaggttct cggtacccag
      421 atctacacca gaagaatgat gatgatgatg gtgcatatgt atatctcctt cttaaagtta
      481 aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt
      541 attaatttcg cgggatcgag atctcgatcc tctacgccgg acgcatcgtg gccggcatca
      601 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
      661 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
      721 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
      781 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
      841 gtcgagatcc cggacaccat cgaatggcgc aaaacctttc gcggtatggc atga
//
