LOCUS       sequence-51732-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MPDZ
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(377..397)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(422..439)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             450..472
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    487..511
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(511..530)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(512..530)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(614..633)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcgg atccgtatcc acctttactg tcaaacgtaa gtactgattc
      121 ttccaacttc caaggttact gtgcctaggg aacactttag caaagcggca accgcttctt
      181 gggtggcatt acgaacgtct tccccattca ccattaatat ctggtctccc tgcatcagtc
      241 ttccatcggc atctgcaatt cctcctttga caatgtctga cacaaatact ccagtatcgt
      301 ttcttttacc aacaatactt aatcctaggc cttttcccgg cttcttctgc agcccaatag
      361 tgagggtgtc actcatggat tggaagtaca ggttctcggt acccagatct acaccagaag
      421 aatgatgatg atgatggtgc atatgtatat ctccttctta aagttaaaca aaattatttc
      481 tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg
      541 atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      601 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      661 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      721 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      781 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg agatcccgga
      841 caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga tagcg
//
