LOCUS       sequence-51734-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MPDZ
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..108
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             447..464
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(517..535)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      531..578
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(702..721)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tccctctgaa tattttgttt actttaagaa ggagatatac atatgcacca tcatcatcat
       61 cattcttctg gtgtagatct gggtaccgag aacctgtact tccaatccat gagtgacacc
      121 ctcactattg ggctgcagaa gaagccggga aaaggcctag gattaagtat tgttggtaaa
      181 agaaacgata ctggagtatt tgtgtcagac attgtcaaag gaggaattgc agatgccgat
      241 ggaagactga tgcagggaga ccagatatta atggtgaatg gggaagacgt tcgtaatgcc
      301 acccaagaag cggttgccgc tttgctaaag tgttccctag gcacagtaac cttggaagtt
      361 ggaagaatca gtacttacgt ttgacagtaa aggtggatac ggatccgaat tcgagctccg
      421 tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
      481 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      541 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      601 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      661 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      721 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
      781 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
      841 ttcacgtagt gggccatcgc ctgaaacgtt tcgcttgctt gcccctt
//
