LOCUS       sequence-51735-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DLG2
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(385..405)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(430..447)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             458..480
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    495..519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(519..538)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(520..538)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(622..641)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc gtatccacct ttactgtcaa taaatggtag tgggtttgcc
      121 aacttttaaa taaactacct ctgatgtgtt ctttaatatt gctactgcct cttcgtgtgt
      181 tacttcttct aaactgtagt tgtttaccat tagtagtcta tctcctactt gcaaccttcc
      241 atctttttgt gcagctcctc catctataat tttagttaca taaatgctgt tgtctccagg
      301 aatgtgttgg ttccccacac ctcctgcaat actgaagcct aaacctttag ggcctttgaa
      361 cagtttgatt tccacaacgg tcatggattg gaagtacagg ttctcggtac ccagatctac
      421 accagaagaa tgatgatgat gatggtgcat atgtatatct ccttcttaaa gttaaacaaa
      481 attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag tcgtattaat
      541 ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg
      601 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
      661 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
      721 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
      781 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag
      841 atcc
//
