LOCUS       sequence-51742-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MPDZ
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(417..437)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(462..479)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             490..512
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    527..551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(551..570)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(552..570)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(654..673)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atccgtatcc acctttactg tcaaagacga
      121 gtacttataa tgctctgtac cataaagact acagggttgc ctgctttccg aatggcttcc
      181 acagcttgtt catggcttgc atctctgagg tccattccat ccacctctac gattctatct
      241 ccaggtttca aggttccatt tttgccagct ggactatctt ccagaacatg tttgatgaaa
      301 atgcccctca tcacttctcc attgcttagc cgactcccca tccctcgtcc accaacaatg
      361 ctgatgccta aggatttgct tggttctctc cagagttcca cccgcctggg ctgcatggat
      421 tggaagtaca ggttctcggt acccagatct acaccagaag aatgatgatg atgatggtgc
      481 atatgtatat ctccttctta aagttaaaca aaattatttc tagaggggaa ttgttatccg
      541 ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatct cgatcctcta
      601 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
      661 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
      721 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
      781 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
      841 aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa
      901 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtg
//
