LOCUS       sequence-51748-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Hoffman LM, Garcha K, Karamboulas K, Cowan MF, Drysdale LM, Horton 
            WA, Underhill TM
  TITLE     BMP action in skeletogenesis involves attenuation of retinoid 
            signaling.
  JOURNAL   J Cell Biol. 2006 Jul 3. 174(1):101-13.
  PUBMED    16818722
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2006 Jul 3. 174(1):101-13."
COMMENT     Sequence Label: pGL3-RARE-luciferase
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        147..343
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      194..329
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      373..382
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(445..463)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tctctatcga taggtaccga gctcggtgaa ctttcggtga accctaccct cgagtgaact
       61 ttcggtgaac cctaccctcg agggtagggt tcaccgaaag ttcactcgac gaattccagt
      121 ctagcccggg ctcgagatct gcgatctgca tctcaattag tcagcaacca tagtcccgcc
      181 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatcg
      241 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
      301 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gcattccggt
      361 actgttggta aagccaccat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc
      421 tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc
      481 ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct
      541 gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca
      601 aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc
      661 gcgttattta tcggagttg
//
