LOCUS       sequence-51753-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MAP3K10
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(146..166)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(191..208)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             219..241
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    256..280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(280..299)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(281..299)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(383..402)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        608..685
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(705..724)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tccaccagcc ctcgtcgccg gacaccgcac agtcttggga aagcacctgg acgcgatcgc
       61 ccctccgcag ggtcagctcc tcgtcgcccg ccgcctcgta gtcgaacacc gcggtccaga
      121 cgggccccgc gggggtcgtg cccatggatt ggaagtacag gttctcggta cccagatcta
      181 caccagaaga atgatgatga tgatggtgca tatgtatatc tccttcttaa agttaaacaa
      241 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa
      301 tttcgcggga tcgagatctc gatcctctac gccggacgca tcgtggccgg catcaccggc
      361 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
      421 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
      481 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
      541 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
      601 gatcccggac accatcgaat ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga
      661 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta
      721 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac
//
